GABA X2 multiplet measured pre- and post administration of vigabatrin in human brain
GABA X2 multiplet measured pre- and post administration of vigabatrin in human brain
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DOI：
10.1002/mrm.10265
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发表时间：
2002-10-01
影响因子：
3.3
通讯作者：
Allen, PS
中科院分区：
文献类型：
--
作者：
Hanstock, CC;Coupland, NJ;Allen, PS
这项工作表明，在溶液和人脑中，在3特斯拉下，GABA的A（2）M（2）X（2）质子自旋系统在2.315 ppm处的X-2-多重峰可以容易地从重叠背景的X-2-多重峰中分辨出来，特别是谷氨酸多重峰，即，谷氨酸AMNPQ自旋系统的PO多重峰。在实验之前，最大化GABA-X-2与其背景（即，168 ms和28 ms）。通过计算整个TE/TM空间中所有参与STEAM序列的代谢物的光谱响应进行测定。从8名正常对照受试者的顶枕叶皮质中3 x 3 x 3 cm（3）体积获得的光谱估计，基线GABA浓度（平均值+/-平均值的标准差（SD））为0.78 +/- 0.04 mM。还在施用50mg.kg-1）氨己烯酸的单剂量后24小时研究了8名对照受试者中的5名。五个中的四个显示出GABA在其基线水平的15-120%的范围内增加。(C)2002 Wiley-Liss，Inc.
This work demonstrates, in solution and in human brain at 3 tesla, that the X-2-multiplet of the A(2)M(2)X(2) proton spin system of GABA at 2.315 ppm can be readily resolved from that of the overlapping background, particularly the glutamate multiplet, i.e., the PO multiplet of the glutamate AMNPQ spin system. Prior to experiment, the values of the stimulated echo acquisition mode (STEAM) sequence parameters TE and TM that maximized the GABA-X-2 discrimination from its background (i.e., 168 ms and 28 ms, respectively) were determined numerically. The determination was made by calculating the spectral response of all contributing metabolites to the STEAM sequence throughout TE/TM space. A baseline GABA concentration (mean +/- standard deviation (SD) of the mean) of 0.78 +/- 0.04 mM was estimated from spectra acquired from a 3 x 3 x 3 cm(3) volume in the parieto-occipital cortex of eight normal control subjects. Five of the eight control subjects were also studied 24 hr post-administration of a single dose of 50 mg.kg(-1) vigabatrin. Four of the five showed increases in GABA in the range of 15-120% of their baseline level. (C) 2002 Wiley-Liss, Inc.