Determination of serum cholestanol by semi-micro high-performance liquid chromatography with electrochemical detection
Determination of serum cholestanol by semi-micro high-performance liquid chromatography with electrochemical detection
复制标题
电化学检测半微量高效液相色谱法测定血清胆甾醇
DOI：
10.1002/bmc.1332
复制
发表时间：
2010
期刊：
影响因子：
--
通讯作者：
F. Kusu
中科院分区：
文献类型：
--
作者：
K. Hojo;H. Hakamata;A. Takahashi;Y. Y. Hosokawa;F. Kusu
建立了一种简便、灵敏、不需衍生化的高效液相色谱电化学检测法（HPLC-ECD）测定胆甾烷醇的方法。电流峰高与胆甾烷醇进样量在1 ~ 200 μM范围内呈线性关系（r= 0.999）。胆甾烷醇的检出限（S/N = 3）为0.23 μM（1.2 pmol）。用本法测定了人和小鼠血清中的总胆甾烷醇，回收率大于90%，RSD（n= 5）小于7.3%。此外，该方法已成功应用于监测喂食高胆固醇饮食的小鼠（一种肌腱黄瘤病（CTX）动物模型）的实验性高胆固醇血症。总之，我们发现这种方法是既简单又有用的测定血清中的胆甾烷醇，有助于CTX的诊断。版权所有© 2009约翰威利父子有限公司。
A simple and sensitive method that dose not require derivatization for determining cholestanol has been developed using HPLC with electrochemical detection (HPLC‐ECD). The current peak height was linearly related to the amount of cholestanol injected, ranging from 1 to 200 μM (r= 0.999). The detection limit (S/N = 3) of cholestanol was 0.23 μM (1.2 pmol). Total cholestanol in control human and mouse serum was determined by the present method with a recovery rate of more than 90% and an RSD (n= 5) of less than 7.3%. Further, this method was successfully applied to monitor experimental hypercholestanolemia in mice fed a high‐cholestanol diet, an animal model of cerebrotendinous xanthomatosis (CTX). In conclusion, we found this method to be both simple and useful for the determination of cholestanol in serum, helping in the diagnosis of CTX. Copyright © 2009 John Wiley & Sons, Ltd.