OXYGEN AND TOXICITY INHIBITION OF AMINO-ACID BIOSYNTHESIS
OXYGEN AND TOXICITY INHIBITION OF AMINO-ACID BIOSYNTHESIS
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DOI：
10.1038/262418a0
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发表时间：
1976-01-01
期刊：
影响因子：
64.8
通讯作者：
BROWN, OR
中科院分区：
文献类型：
--
作者：
BOEHME, DE;VINCENT, K;BROWN, OR
尽管氧气对多种人类疾病具有治疗作用，但其用途受到其毒性的限制1,2。尽管取得了进展，但在暴露于高压氧的人类和其他动物中观察到的抽搐原因仍然未知3,4，并且对长期暴露于升高的氧张力导致的肺损伤的生化基础知之甚少5。氧毒性虽然必然以不同的方式表达，但影响从细菌到人类的各种生命形式3,4。对于细胞和亚细胞水平上共有的毒性机制有相当多的理论基础和实验证据，并且对毒性基本位点的研究通常涉及组织培养物或细菌2。微量盐培养基中的高压氧可快速但可逆地抑制大肠杆菌的生长和呼吸6。通过营养补充剂（包括酵母提取物）可以显着保护某些微生物免受高压氧的生长抑制作用7。我们现在发现E.大肠杆菌E-26（其来源之前已被确定6）由于其氨基酸含量而受到酵母提取物的保护，免受氧毒性：20种氨基酸的混合物提供了类似的保护。
ALTHOUGH oxygen is of therapeutic benefit for various human diseases, its usefulness is limited by its toxicity1,2. In spite of progress, the cause of the convulsions observed in humans and other animals exposed to hyperbaric oxygen remains unknown3,4, and the biochemical basis of the lung damage resulting from chronic exposure to increased oxygen tensions is poorly understood5. Oxygen toxicity, although necessarily expressed in different ways, affects various life forms from bacteria to man3,4. There is considerable theoretical basis and experimental evidence for mechanisms of toxicity shared at the cellular and subcellular levels, and research on basic sites of toxicity has often involved tissue cultures or bacteria2. The growth and respiration ofEscherichia coliis rapidly, but reversibly, inhibited by hyperbaric oxygen in minimal salts medium6. Significant protection for some microbes against the growth-inhibitory effects of hyperbaric oxygen has been obtained with nutritional supplements, including yeast extract7. We have now found thatE. coliE-26, the source of which has been identified previously6, is protected from oxygen toxicity by yeast extract because of its amino acid content: a mixture of 20 amino acids gave similar protection.