Glutathione S-transferases as antioxidant defence agents confer pyrethroid resistance in Nilaparvata lugens
Glutathione S-transferases as antioxidant defence agents confer pyrethroid resistance in Nilaparvata lugens
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DOI：
10.1042/0264-6021:3570065
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发表时间：
2001-07-01
影响因子：
4.1
通讯作者：
Hemingway, J
中科院分区：
文献类型：
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作者：
Vontas, JG;Small, GJ;Hemingway, J
用拟除虫菊酯二氯苯醚菊酯和高效氯氟氰菊酯选择褐飞虱实验室种群，增加了其对这两种杀虫剂的抗性。生化分析和代谢抑制剂的协同作用研究表明，谷胱甘肽S-转移酶（GST）的主要过氧化物酶活性的升高赋予抗性的拟除虫菊酯，而酯酶赋予的部分抗性氯菊酯。纯化的酯酶水解氯菊酯在一个缓慢的速度，但无论是拟除虫菊酯或其主要代谢产物与部分纯化的GST的孵育没有影响的代谢概况。尽管GST对两种拟除虫菊酯的抑制作用敏感，但如先前假设的那样，它们不作为结合蛋白[Grant和Matsumura（1988）Insect Biochem.18，615-622]。我们证明，拟除虫菊酯，除了其神经毒性作用，诱导昆虫的氧化应激和脂质过氧化。拟除虫菊酯暴露诱导脂质过氧化物、蛋白质氧化和还原型谷胱甘肽耗竭。抗性菌株中GST升高可减弱拟除虫菊酯诱导的脂质过氧化作用并降低死亡率，而其体内抑制作用消除了其保护作用。因此，我们推测GST升高在赋予N. lugens是通过保护组织免受氧化损伤。我们的研究将GST的功效范围扩展到拟除虫菊酯杀虫剂，并可能解释GST升高在其他拟除虫菊酯抗性昆虫中的作用。
Selection of a laboratory colony of the brown planthopper Nilaparvata lugens with the pyrethroids permethrin and lambda -cyhalothrin increased its resistance to both insecticides. Biochemical analysis and synergistic studies with metabolic inhibitors indicated that elevated glutathione S-transferases (GSTs) with a predominant peroxidase activity conferred resistance to both pyrethroids, whereas esterases conferred part of the resistance to permethrin. Purified esterases hydrolysed permethrin at a slow rate, but incubation of either pyrethroid or their primary metabolites with partially purified GSTs had no effect on the metabolic profile. Although GSTs were sensitive to inhibition by both pyrethroids, they did not serve as binding proteins, as previously hypothesized [Grant and Matsumura (1988) Insect Biochem. 18, 615-622]. We demonstrate that pyrethroids, in addition to their neurotoxic effect, induce oxidative stress and lipid peroxidation in insects. Pyrethroid exposure induced lipid peroxides, protein oxidation and depleted reduced glutathione. Elevated GSTs in the resistant strains attenuated the pyrethroid-induced lipid peroxidation and reduced mortality, whereas their in vivo inhibition eliminated their protective role. We therefore hypothesize that the main role of elevated GSTs in conferring resistance in N. lugens is through protecting tissues from oxidative damage. Our study extends the GSTs range of efficacy to pyrethroid insecticides and possibly explains the role of elevated GSTs in other pyrethroid-resistant insects.