Asymmetric synthesis of chiral cyclic amine from cyclic imine by bacterial whole-cell catalyst of enantioselective imine reductase
Asymmetric synthesis of chiral cyclic amine from cyclic imine by bacterial whole-cell catalyst of enantioselective imine reductase
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DOI：
10.1039/c0ob00353k
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发表时间：
2010-01-01
影响因子：
3.2
通讯作者：
Nagasawa, Toru
中科院分区：
文献类型：
--
作者：
Mitsukura, Koichi;Suzuki, Mai;Nagasawa, Toru
利用2-甲基-1-吡咯啉（2-MPN）筛选，发现Streptomyces sp. GF3587和3546是具有高R-和s -选择性的亚胺还原菌株。他们的全细胞催化剂在葡萄糖存在下分别从2-MPN以91-92%的转化率产生91 mM r -2-甲基吡啶（R-2-MP）和27.5 mM S-2-MP （92.3% e.e.）。
Streptomyces sp. GF3587 and 3546 were found to be iminereducing strains with high R- and S-selectivity by screening using 2-methyl-1-pyrroline (2-MPN). Their whole-cell catalysts produced 91 mM R-2-methylpyrrolidine (R-2-MP) with 99.2% e.e. and 27.5 mM S-2-MP (92.3% e.e.) from 2-MPN at 91-92% conversion in the presence of glucose, respectively.