THE EFFECT OF VARIOUS ANTIOXIDANTS AND OTHER MODIFYING AGENTS ON OXYGEN-RADICAL-GENERATED DNA-DAMAGE IN HUMAN-LYMPHOCYTES IN THE COMET ASSAY
THE EFFECT OF VARIOUS ANTIOXIDANTS AND OTHER MODIFYING AGENTS ON OXYGEN-RADICAL-GENERATED DNA-DAMAGE IN HUMAN-LYMPHOCYTES IN THE COMET ASSAY
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DOI：
10.1016/0027-5107(94)90300-x
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发表时间：
1994-05-01
期刊：
影响因子：
--
通讯作者：
SCHMEZER, P
中科院分区：
文献类型：
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作者：
ANDERSON, D;YU, TW;SCHMEZER, P
使用COMET试验研究了抗氧化剂和各种其他修饰剂对人淋巴细胞中氧自由基产生的DNA损伤的影响。过氧化氢（H2O2）和博莱霉素（BLM）产生了明确的剂量相关反应。在38项独立实验中，研究中使用的两个供体之间的阴性和阳性对照数据具有一致性。内源性抗氧化剂过氧化氢酶废除与H2O2的影响，但只有轻微的影响与BLM的反应。超氧化物歧化酶没有改变与H2O2的反应，只有轻微影响BLM。外源性抗氧化剂维生素C本身产生了明显的剂量相关反应。与H2O2联合给药时，低剂量时有较小的保护作用，高剂量时有加重作用，但这些都在实验间变异性范围内。维生素E（trolox）与H2O2或BLM或其本身均无影响。水飞蓟素保护免受H2O2的影响。其他修饰剂，如脱辅基转铁蛋白和甲磺酸去铁胺产生了明确的剂量相关的保护作用，由于BLM。由于H2O2，这种保护作用较小。在氯化亚铁的存在下，由于BLM的影响加剧。在6名吸烟者和6名非吸烟者的小样本中，与非吸烟者相比，吸烟者的反应接近临界显著性（P=0.054）。这些观察结果表明，COMET试验是一个有用的工具，检查有关的问题，在人类淋巴细胞中的氧化应激。
The effects of antioxidants and various other modifying agents on oxygen-radical-generated DNA damage in human lymphocytes have been investigated using the COMET assay. Hydrogen peroxide (H2O2) and bleomycin (BLM) have produced clear dose-related responses. In 38 independent experiments, there was consistency between the two donors used in the study for the negative and positive control data. The endogenous antioxidant catalase abolished effects with H2O2, but only slightly affected the response with BLM. Superoxide dismutase did not alter the response with H2O2 and only slightly affected BLM. The exogenous antioxidant vitamin C produced a clear dose-related response on its own. In combination with H2O2, there were small protective effects at low doses and exacerbating effects at high doses, but these were within the inter-experimental variability range. Vitamin E (trolox) produced no effects with either H2O2 or BLM, or on its own. Silymarin protected against the effect due to H2O2. Other modifying agents such as apo-transferrin and deferoxamine mesylate produced a clear dose-related protection of effects due to BLM. This protection was less due to H2O2. In the presence of ferrous chloride, the effect due to BLM was exacerbated. In a small sample of 6 smokers and 6 non-smokers, responses from smokers approached borderline significance (P=0.054) by comparison with non-smokers. These observations would suggest that the COMET assay is a useful tool for examining issues related to oxidative stress in human lymphocytes.