In vitro cytostatic and immunomodulatory properties of the medicinal mushroom Lentinula edodes
In vitro cytostatic and immunomodulatory properties of the medicinal mushroom Lentinula edodes
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DOI：
10.1016/j.phymed.2007.11.029
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发表时间：
2008-06-01
期刊：
影响因子：
7.9
通讯作者：
Harding, S. E.
中科院分区：
文献类型：
--
作者：
Israilides, C.;Kletsas, D.;Harding, S. E.
香菇，又名香菇，是东方广泛使用的药用蘑菇之一。这种蘑菇提取物的抗肿瘤活性已经在动物和人类中得到了广泛的证明。然而，这种活性是由宿主介导的，而不是通过对癌细胞的直接细胞毒活性来实现的。本研究用四甲基偶氮唑盐细胞毒试验证实了香菇水提物对人乳腺癌细胞株MCF-7的细胞毒性和细胞生长抑制(细胞抑制)作用。子实体和菌丝体提取液对正常细胞无明显抑制作用，不同之处在于后者对正常细胞无明显抑制。此外，根据DNA合成分析，菌丝体提取物在癌细胞和正常细胞系中都没有诱导任何细胞抑制作用。癌细胞的IC50值相对较低，而正常成纤维细胞的IC50值较高，反映出癌细胞的增殖受到明显抑制。通过淋巴细胞转化试验(LTT)检测了子实体和菌丝体提取物的免疫刺激活性，该试验是基于活性免疫调节剂在体外增强大鼠胸腺细胞对T有丝分裂原的增殖反应。子实体制剂和菌丝体制剂都能直接促进大鼠胸腺细胞的增殖，并在T-丝裂原PHA存在的情况下起到协同刺激作用。有趣的是，这两种提取物类似于酵母多糖，显示SIcomit/Simit比率约为2，表明具有佐剂特性。总体而言，香菇水提物在体外直接抑制人乳腺癌细胞的增殖，并在体外表现出促有丝分裂和共促有丝分裂活性的免疫刺激特性。(C)2007年爱思唯尔股份有限公司。版权所有。
Lentinula edodes, known as "shiitake" is one of the widely used medicinal mushrooms in the Orient. Antitumour activity of extracts of this mushroom has been widely demonstrated in animals and humans. However, this activity was shown to be host mediated and not by direct cytotoxic activity to cancer cells. This study demonstrates cytotoxic and cell growth inhibitory (cytostatic) effect of aqueous extracts of the mushroom on MCF-7 human breast adenocarcinoma cell line using an MTT cytotoxicity assay. Such effect was demonstrated with fruit body and mycelial extracts, the difference being that there was no significant suppression on normal cells with the latter. Furthermore mycelial extracts did not induce any cytostatic effect in both cancer and normal cell lines based on a DNA synthesis assay. The significant suppression of the proliferation of cancer cells was reflected by the comparatively low IC50 values and the simultaneous higher respective values on normal fibroblast cells. The immunostimulatory activity of both fruit body and mycelial extracts was tested by the lymphocyte transformation test (LTT), which is based on the capacity of active immunomodulators to augment the proliferative response of rat thymocytes to T mitogens in vitro. Both fruit body and mycelial preparations were able to enhance the proliferation of rat thymocytes directly and act as co-stimulators in the presence of the T-mitogen PHA. Interestingly both extracts, similarly to zymosan showed SIcomit/SImit ratios of about 2, indicating adjuvant properties. Overall L. edodes aqueous extracts have demonstrated direct inhibition of the proliferation of human breast cancer cells in vitro and immunostimulatory properties in terms of mitogenic and co-mitogenic activity in vitro. (c) 2007 Elsevier GmbH. All rights reserved.