Spatiotemporal quantification of cerebral blood flow during functional activation in rat somatosensory cortex using laser-speckle flowmetry
Spatiotemporal quantification of cerebral blood flow during functional activation in rat somatosensory cortex using laser-speckle flowmetry
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DOI：
10.1097/00004647-200405000-00005
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发表时间：
2004-05-01
影响因子：
6.3
通讯作者：
Greenberg, JH
中科院分区：
文献类型：
--
作者：
Durduran, T;Burnett, MG;Greenberg, JH
用激光散斑流量术研究电刺激大鼠前、后爪后的激活流耦合。以高的横向空间(Um)和时间(Msec)分辨率对功能激活进行量化。定量研究了不同的激活水平和刺激持续时间，并与以前的激光多普勒测量结果一致。有趣的是，反应的大小而不是整体形状被发现与刺激幅度和离激活质心的距离成比例。这些结果为脑血流对功能激活反应的空间特征提供了新的见解，该方法将有助于更好地理解正常和病理条件下神经元活动和血流动力学的耦合。
Laser-speckle flowmetry was used to characterize activation flow coupling after electrical somatosensory stimulation of forepaw and hindpaw in the rat. Quantification of functional activation was made with high transverse spatial (mum) and temporal (msec) resolution. Different activation levels and duration of stimulation were quantitatively investigated, and were in good agreement with previous laser-Doppler measurements. Interestingly, the magnitude but not the overall shape of the response was found to scale with stimulus amplitude and the distance from the activation centroid. The results provide new insights about the spatial characteristics of cerebral blood flow response to functional activation, and the method should lead to improved understanding of the coupling of neuronal activity and hemodynamics under normal and pathologic conditions.