Persistence of allografts in the peritoneal cavity after prenatal transplantation in mice.
Persistence of allografts in the peritoneal cavity after prenatal transplantation in mice.
复制标题
小鼠产前移植后同种异体移植物在腹腔内的持久性。
DOI：
10.1111/j.1537-2995.2007.01570.x
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发表时间：
2008
期刊：
影响因子：
2.9
通讯作者：
Muench,MarcusO
中科院分区：
文献类型：
--
作者：
Chen,Jeng-Chang;Chang,Ming-Ling;Muench,MarcusO
背景：子宫内移植（IUT）通常通过腹腔注射进行，但关于移植后第一周内腹腔移植细胞命运的信息很少。 研究设计和方法：胎儿移植是在 B6D2F1（C57BL/6 × DBA/2，H-2b/d）中进行的 C57BL/6（H-2b） 通过腹腔注射低密度骨髓细胞在胎龄 13 天时进行小鼠品系组合。成年C57BL/6小鼠作为出生后移植组。采用流式细胞术检测腹膜、外周血和各种造血组织中的供体细胞水平。结果：胎儿移植后4周内可检测到腹膜嵌合度，范围为0.02%～23.2%，其中移植后2周嵌合度中位数最高。外周血、脾脏、骨髓、肝脏和胸腺中的供体细胞通常较低（<0.2%），移植后 2 周时供体细胞水平有升高的趋势。胎儿腹膜的植入主要是骨髓细胞和 B 细胞，少量 T 细胞。相比之下，移植单倍体骨髓的成年小鼠在移植后一周内几乎清除了腹膜中的所有供体细胞。没有证据表明供体细胞在成人移植后曾迁移到任何造血组织。结论：尽管造血组织中的植入有限，但胎儿移植后供体细胞在腹膜中持续存在2周或更长时间。胎儿腹膜可以作为胎儿外来细胞的避难所，这可能有助于开发针对出生缺陷的新型细胞疗法。
BACKGROUND:In utero transplantation (IUT) is usually performed by intraperitoneal injection, but there is little information regarding the fate of intraperitoneally transplanted cells in the first weeks after transplantation.STUDY DESIGN AND METHODS:Fetal transplantation was performed in a B6D2F1(C57BL/6 × DBA/2, H‐2b/d) into C57BL/6 (H‐2b) murine strain combination at the gestational age of 13 days by intraperitoneal injection of light‐density marrow cells. Adult C57BL/6 mice were used as the postnatal transplantation group. Donor cell levels in the peritoneum, peripheral blood, and various hematopoietic tissues were examined by flow cytometry.RESULTS:After fetal transplantation, peritoneal chimerism could be detected for 4 weeks, ranging from 0.02 to 23.2 percent with the highest median chimerism at 2 weeks after transplantation. Donor cells in the peripheral blood, spleen, marrow, liver, and thymus were usually low (<0.2%) with a tendency for higher donor cell levels at 2 weeks after transplantation. Engraftment in fetal peritoneum was primarily by myeloid and B cells with few T cells. In contrast, adult mice transplanted with haplogeneic marrow cleared nearly all donor cells from the peritoneum within a week of transplant. There was no evidence showing that donor cells had ever migrated to any hematopoietic tissues after adult transplantation.CONCLUSION:Despite limited engraftment in hematopoietic tissues, donor cells persisted in the peritoneum for 2 weeks or longer after fetal transplantation. The fetal peritoneum may serve as a sanctuary for foreign cells in the fetus, which may aid the development of novel cellular therapies for birth defects.