HYPOTHESIS FOR THE EVOLUTION OF 3-HELIX CHL A/B AND CHL A/C LIGHT-HARVESTING ANTENNA PROTEINS FROM 2-HELIX AND 4-HELIX ANCESTORS
HYPOTHESIS FOR THE EVOLUTION OF 3-HELIX CHL A/B AND CHL A/C LIGHT-HARVESTING ANTENNA PROTEINS FROM 2-HELIX AND 4-HELIX ANCESTORS
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DOI：
10.1007/bf00029382
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发表时间：
1994-02-01
影响因子：
3.7
通讯作者：
PICHERSKY, E
中科院分区：
文献类型：
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作者：
GREEN, BR;PICHERSKY, E
光合真核生物的核编码Chi nib和Chi ale天线蛋白是蛋白质大家族的一部分，该家族还包括早期光诱导蛋白（ELIP）和PSII的22 kDa内在蛋白（由psbS基因编码）。这个家族的所有成员都有三个跨膜螺旋，除了psbS蛋白，它有四个。将这些蛋白质的氨基酸序列进行比较，并将其与豌豆LHC II I型的三维结构相关联（Kuhlbrandt和Wang，Nature 350：130-134，1991）。psbS家族的三螺旋成员的相似性表明，后者来自一个四螺旋祖先，失去了其C-末端螺旋删除。psbS蛋白的两个半部分之间的强烈内部相似性表明，它反过来又是编码双螺旋蛋白的基因复制的结果。由于psbS被报道存在于至少一种蓝细菌中，因此祖先四螺旋蛋白可能在引起光合真核生物的内共生事件或事件之前就已经存在。Chi alb和Chi ale天线蛋白以及红藻中的免疫相关蛋白可能有一个共同的祖先，该祖先存在于早期的光合真核生物中，并且早于它们分化为红藻、有色植物和绿藻谱系。LHC I-LHC II的分化可能发生在高等植物与绿藻和裸藻分离之前，而不同类型的LHC I和LHC II蛋白质则出现在被子植物和裸子植物分离之前。
The nuclear-encoded Chi nib and Chi ale antenna proteins of photosynthetic eukaryotes are part of an extended family of proteins that also includes the early light-induced proteins (ELIPs) and the 22 kDa intrinsic protein of PSII (encoded by psbS gene). All members of this family have three transmembrane helices except for the psbS protein, which has four. The amino acid sequences of these proteins are compared and related to the three-dimensional structure of pea LHC II Type I (Kuhlbrandt and Wang, Nature 350: 130-134, 1991). The similarity of psbS to the three-helix members of the family suggests that the latter arose from a four-helix ancestor that lost its C-terminal helix by deletion. Strong internal similarity between the two halves of the psbS protein suggests that it in turn arose as the result of the duplication of a gene encoding a two-helix protein. Since psbS is reported to be present in at least one cyanobacterium, the ancestral four-helix protein may have been present prior to the endosymbiotic event or events that gave rise to the photosynthetic eukaryotes. The Chi alb and Chi ale antenna proteins, and the immunologically-related proteins in the rhodophytes may have had a common ancestor which was present in the early photosynthetic eukaryotes, and predated their division into rhodophyte, chromophyte and chlorophyte lineages. The LHC I-LHC II divergence probably occurred before the separation of higher plants from chlorophyte algae and euglenophytes, and the different Types of LHC I and LHC II proteins arose prior to the separation of angiosperms and gymnosperms.