Identification of novel long non-coding RNAs in triple-negative breast cancer.
Identification of novel long non-coding RNAs in triple-negative breast cancer.
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三阴性乳腺癌中新型长非编码RNA的鉴定
DOI：
10.18632/oncotarget.4419
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发表时间：
2015-08-28
期刊：
影响因子：
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通讯作者：
Cao F
中科院分区：
文献类型：
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作者：
Shen X;Xie B;Ma Z;Yu W;Wang W;Xu D;Yan X;Chen B;Yu L;Li J;Chen X;Ding K;Cao F
三阴性乳腺癌（TNBC）的特点是预后特别差，并且没有与预后显著相关的既定标志物。长链非编码RNA（longnon-codingRNA，lncRNA）是一类在肿瘤生物学中起重要作用的非编码RNA。然而，关于它们在TNBC发病机制中的机制作用知之甚少。本研究利用Agilent Human lncRNA芯片检测了TNBC组织及相应正常组织中lncRNA的表达模式。我们鉴定了1，758种lncRNA和1，254种mRNA的差异表达（≥ 2倍变化），表明许多lncRNA在TNBC中显著上调或下调。其中，XR_250621.1和NONHSAT 125629分别是上调和下调最多的lncRNA。采用qRT-PCR验证微阵列分析结果，结果与微阵列数据一致。通过GO和KEGG通路分析，初步确定了TNBC发病机制中的一些通路，包括微管运动和DNA复制。我们的研究揭示了一组lncRNA在TNBC组织中的差异表达，表明它们可能在TNBC中起作用。这些结果揭示了lncRNA的生物学功能，并为探索乳腺癌的潜在治疗靶点提供了有用的信息。
Triple-negative breast carcinomas (TNBC) are characterized by particularly poor outcomes, and there are no established markers significantly associated with prognosis. Long non-coding RNAs (lncRNAs) are subclass of noncoding RNAs that have been recently shown to play critical roles in cancer biology. However, little is known about their mechanistic role in TNBC pathogenesis. In this report, we investigated the expression patterns of lncRNAs from TNBC tissues and matched normal tissues with Agilent Human lncRNA array. We identified 1,758 lncRNAs and 1,254 mRNAs that were differentially expressed (≥ 2-fold change), indicating that many lncRNAs are significantly upregulated or downregulated in TNBC. Among these, XR_250621.1 and NONHSAT125629 were the most upregulated and downregulated lncRNAs respectively. qRT-PCR was employed to validate the microarray analysis findings, and results were consistent with the data from the microarrays. GO and KEGG pathway analysis were applied to explore the potential lncRNAs functions, some pathways including microtubule motor activity and DNA replication were identified in TNBC pathogenesis. Our study revealed that a set of lncRNAs were differentially expressed in TNBC tissues, suggesting that they may play role in TNBC. These results shed light on lncRNAs’ biological functions and provide useful information for exploring potential therapeutic targets for breast cancer.