Segregation of genomes in polyploid tumour cells following mitotic catastrophe
Segregation of genomes in polyploid tumour cells following mitotic catastrophe
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DOI：
10.1016/j.cellbi.2005.10.008
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发表时间：
2005-12-01
影响因子：
3.9
通讯作者：
Illidge, TM
中科院分区：
文献类型：
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作者：
Erenpreisa, J;Kalejs, M;Illidge, TM
照射后，p53功能缺陷的肿瘤细胞经历有丝分裂灾难并形成内多倍体细胞。这些细胞中的一小部分分离细胞核，并产生有活力的后代。在这里，我们在五个肿瘤细胞系中研究了这一过程。有丝分裂失败后，肿瘤细胞进入内循环，形成单核或多核巨细胞（MOGC和MNGC）。MNGC起源于停滞的后期，MOGC起源于停滞的中期。在这两种情况下，个体基因组通过与单个微管组织中心的联系建立放射状模式。基因组的分离也是有序的。MNGC表现为有丝分裂后期恢复的特征。在MOGC中，亚核保留堆叠的中期板排列，并被核被膜的褶皱分开。有丝分裂然后直接从中期在亚核中恢复。所提供的数据表明，内多倍体肿瘤细胞保留了个体基因组的完整性，并可能从中断的点重新启动有丝分裂。(c)2005年国际细胞生物学联合会。由爱思唯尔有限公司出版。保留所有权利。
Following irradiation p53-function-deficient tumour cells undergo mitotic catastrophe and form endopolyploid cells. A small proportion of these segregates nuclei, and give rise to viable descendants. Here we studied this process in five tumour cell lines. After mitotic failure, tumour cells enter the endocycle and form mono-nucleated or multi-nucleated giant cells (MOGC and MNGC). MNGC arise from arrested anaphases, MOGC, from arrested metaphases. In both cases the individual genomes establish a radial pattern by links to a single microtubule organizing centre. Segregation of genomes is also ordered. MNGC present features of mitosis being resumed from late anaphase. In MOGC the sub-nuclei retain arrangement of stacked metaphase plates and are separated by folds of the nuclear envelope. Mitosis then resumes in sub-nuclei directly from metaphase. The data presented indicate that endopolyploid tumour cells preserve the integrity of individual genomes and can potentially reinitiate mitosis from the point at which it was interrupted. (c) 2005 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.