Lycopene inhibits experimental metastasis of human hepatoma SK-Hep-1 cells in athymic nude mice
Lycopene inhibits experimental metastasis of human hepatoma SK-Hep-1 cells in athymic nude mice
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DOI：
10.1093/jn/138.3.538
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发表时间：
2008-03-01
影响因子：
4.2
通讯作者：
Hu, Miao-Lin
中科院分区：
文献类型：
--
作者：
Huang, Chin-Shiu;Liao, Jiunn-Wang;Hu, Miao-Lin
番茄红素在体外已被证明能抑制肿瘤转移，但在体内是否具有抗转移作用尚不清楚。在此，裸鼠每周口服补充2次低或高剂量的番茄红素[1或20 mg/kg体重（BW）]或β-胡萝卜素（20 mg/kg BW），持续12周。开始补充后两周，通过尾静脉向小鼠注射一次人肝癌SK-Hep-1细胞。基质金属蛋白酶（MMP）-2和血管内皮生长因子（VEGF）的血浆水平逐渐增加，在肿瘤注射后的肿瘤注射小鼠（肿瘤对照），但番茄红素或β-胡萝卜素的补充显着降低。肿瘤注射后10周，处死小鼠，发现肿瘤转移局限于肺。与肿瘤对照组相比，高番茄红素补充剂使肿瘤的平均数量从14 +/- 8减少到3 +/- 5（P
Lycopene has been shown to inhibit tumor metastasis in vitro, but it is unclear whether lycopene is antimetastatic in vivo. Here, nude mice were orally supplemented 2 times per week for 12 wk with a low or high dose of lycopene [1 or 20 mg/kg body weight (BW)] or with beta-carotene (20 mg/kg BW). Two weeks after the beginning of supplementation, mice were injected once with human hepatoma SK-Hep-1 cells via the tail vein. Plasma levels of matrix metalloproteinase (MMP)-2 and vascular endothelial growth factor (VEGF) increased gradually in tumor-injected mice (tumor controls) following tumor injection but were markedly lowered by lycopene or beta-carotene supplementation. Ten weeks after tumor injection, mice were killed and tumor metastasis was found to be confined to the lungs. Compared with the tumor controls, high-lycopene supplementation lowered the mean number of tumors from 14 +/- 8 to 3 +/- 5 (P