High-resolution electron diffraction of reconstituted PhoE porin.
High-resolution electron diffraction of reconstituted PhoE porin.
复制标题
重组 PhoE 孔蛋白的高分辨率电子衍射。
DOI：
10.1016/0022-2836(88)90393-2
复制
发表时间：
1988
影响因子：
5.6
通讯作者：
Jap,BK
中科院分区：
文献类型：
--
作者：
Jap,BK
PhoE 孔蛋白已用磷脂重组，形成大膜片。电子衍射表明，重组的 PhoE 孔蛋白形成高度相干的晶体阵列，以 3.4 rmÅ 的分辨率提供结构信息。晶体形式为斜方空间群 P2 1 2 1 2，晶胞尺寸 a= 150 A ̊ 和 b= 129 A ̊。负染 PhoE 晶体斑块的图像显示，一个晶胞中有四个 PhoE 孔蛋白三聚体。
PhoE porin has been reconstituted with phospholipid, forming large membrane patches. Electron diffraction shows that the reconstituted PhoE porin forms highly coherent crystalline arrays, giving structural information to a resolution of 3.4 rmÅ. The crystal form is of the orthorhombic space group P2 1 2 1 2, with unit cell dimensions a= 150 A ̊ and b= 129 A ̊. Images of negatively stained PhoE crystalline patches show that there are four PhoE porin trimers in a unit cell.