TRANSCRIPTION OF VACCINIA VIRUS EARLY GENES BY ENZYMES ISOLATED FROM VACCINIA VIRIONS TERMINATES DOWNSTREAM OF A REGULATORY SEQUENCE
TRANSCRIPTION OF VACCINIA VIRUS EARLY GENES BY ENZYMES ISOLATED FROM VACCINIA VIRIONS TERMINATES DOWNSTREAM OF A REGULATORY SEQUENCE
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DOI：
10.1016/0092-8674(86)90702-6
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发表时间：
1986-09-26
期刊：
影响因子：
64.5
通讯作者：
MOSS, B
中科院分区：
文献类型：
--
作者：
ROHRMANN, G;YUEN, L;MOSS, B
我们描述了一个体外转录系统，在该系统中，牛痘病毒mrna的聚腺苷化3‘’末端通过调控信号的下游终止形成。当含有牛痘早期基因的线性DNA模板与从牛痘病毒粒子中提取的可溶性酶一起孵育时，成熟大小的mrna在1分钟内合成，而更长的转录本直到后来才被检测到。删除位于编码区下游的一系列胸苷酸残基可以消除终止。转录本的3”端在病毒或载体衍生的序列中形成异质性，在富t终止信号后的50至70个核苷酸之间。转录本的聚腺苷化不考虑末端序列，并通过添加外源RNA被抑制。虽然外源rna也被聚腺苷化，但没有观察到3'末端的加工。
We describe an in vitro transcription system in which the polyadenylated 3'' ends of vaccinia virus mRNAs are formed by termination downstream of a regulatory signal. When linear DNA templates containing vaccinia early genes were incubated with soluble enzymes extracted from vaccinia virions, mature-size mRNAs were synthesized within 1 min, whereas longer run-off transcripts were not detected until later. Deleting a series of thymidylate residues located downstream of the coding region abolished termination. The 3'' ends of the transcripts formed heterogeneously, between 50 and 70 nucleotides past the T-rich termination signal, in either viral- or vector-derived sequences. Polyadenylation of the transcripts occurred without regard to the terminal sequence and was inhibited by adding exogenous RNA. Although exogenous RNAs were also polyadenylated, processing of 3'' ends was not observed.