Genomic comparison of Salmonella enterica serovars and Salmonella bongori by use of an S-enterica serovar Typhimurium DNA microarray
Genomic comparison of Salmonella enterica serovars and Salmonella bongori by use of an S-enterica serovar Typhimurium DNA microarray
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DOI：
10.1128/jb.185.2.553-563.2003
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发表时间：
2003-01-01
影响因子：
3.2
通讯作者：
Falkow, S
中科院分区：
文献类型：
--
作者：
Chan, K;Baker, S;Falkow, S
沙门氏菌属由超过2，200种血清型组成，尽管它们具有密切的遗传相关性，但它们的宿主范围和致病能力不同。影响每个血清型的宿主适应水平的遗传因素，它们是如何进化或获得的，它们对每个血清型进化的影响，以及血清型之间的遗传关系是非常有趣的，因为它们提供了对宿主范围和疾病进展中这些差异背后的机制的深入了解。我们用鼠伤寒沙门氏菌斑点DNA微阵列对两种沙门氏菌的不同血清型和菌株进行了基因组杂交。肠道沙门氏菌（亚种I和IIIa）和邦戈里沙门氏菌，以深入了解血清型的遗传组织。我们的研究结果与以前发表的DNA关联和多位点酶电泳数据基本一致。我们的发现也揭示了新的信息。我们观察到一个更遥远的关系，血清型亚利桑那州（亚种IIIa）的亚种我血清型比以前测量。我们还观察到亚利桑那州的SPI-2致病岛的变异性，表明它已经从其他血清型不同的方式演变。此外，我们确定了共享的遗传特征的S。伤寒、甲型副伤寒和仙台病毒，它们具有引起人类肠道发热的独特能力。因此，而沙门氏菌的血清群的分类组织提供了一个很好的遗传相关性的第一近似值，我们表明，它不占基因组的变化，有助于血清型的宿主适应程度。
The genus Salmonella consists of over 2,200 serovars that differ in their host range and ability to cause disease despite their close genetic relatedness. The genetic factors that influence each serovar's level of host adaptation, how they evolved or were acquired, their influence on the evolution of each serovar, and the phylogenic relationships between the serovars are of great interest as they provide insight into the mechanisms behind these differences in host range and disease progression. We have used an Salmonella enterica serovar Typhimurium spotted DNA microarray to perform genomic hybridizations of various serovars and strains of both S. enterica (subspecies I and IIIa) and Salmonella bongori to gain insight into the genetic organization of the serovars. Our results are generally consistent with previously published DNA association and multilocus enzyme electrophoresis data. Our findings also reveal novel information. We observe a more distant relationship of serovar Arizona (subspecies IIIa) from the subspecies I serovars than previously measured. We also observe variability in the Arizona SPI-2 pathogenicity island, indicating that it has evolved in a manner distinct from the other serovars. In addition, we identify shared genetic features of S. enterica serovars Typhi, Paratyphi A, and Sendai that parallel their unique ability to cause enteric fever in humans. Therefore, whereas the taxonomic organization of Salmonella into serogroups provides a good first approximation of genetic relatedness, we show that it does not account for genomic changes that contribute to a serovar's degree of host adaptation.