Molecular diversity of Fonsecaea (Chaetothyriales) causing chromoblastomycosis in southern China
Molecular diversity of Fonsecaea (Chaetothyriales) causing chromoblastomycosis in southern China
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DOI：
10.1080/13693780802468209
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发表时间：
2009-01-01
期刊：
影响因子：
2.9
通讯作者：
De Hoog, G. S.
中科院分区：
文献类型：
--
作者：
Xi, Liyan;Sun, Jiufeng;De Hoog, G. S.
着色芽生菌病是一种由毛囊藻目成员引起的慢性皮肤和皮下感染，常见于中国。在病原体中，刚毛藻属的成员在中国北方占主导地位。或者，Fonsecaea spp。在中国南方尤为常见。然而，由于不同物种缺乏独特的形态特征，在中国回收的真菌分离菌的鉴定很困难。因此，通过使用形态学、ITS rDNA 序列多样性以及部分通过使用随机扩增多态性 DNA (RAPD) 分型来重新评估从有症状患者中分离出的 24 株 Fonsecaea 分离株。包括形态突变体在内的 20 个菌株被发现为 Fonsecaea monophora，而 4 个菌株对应于 F. pedrosoi。我们已经证明，Fonsecaea monophora 是中国南方有色芽生菌病的主要病原体，并且其种群表现出明显的地理结构。
Chromoblastomycosis is a chronic, cutaneous and subcutaneous infection caused by members of the order Chaetothyriales and commonly found in China. Among the etiologic agents, members of the genus Cladophialophora are predominant in northern China. Alternatively, Fonsecaea spp. are particularly common in southern China. However, the identification of Fonsecaea isolates recovered in China is difficult due to the fact that different species lack distinctive morphological characters. Therefore, the identification of 24 Fonsecaea isolates from symptomatic patients were re-evaluated by using morphology, ITS rDNA sequence diversity and partly through the use of randomly amplified polymorphic DNA (RAPD) typing. Twenty strains, including a morphological mutant were found to be Fonsecaea monophora, while four strains corresponded to F. pedrosoi. We have demonstrated that Fonsecaea monophora is the predominant etiologic agent of chromoblastomycosis in southern China and populations showed marked geographic structuring.