Physicochemical surface properties of elementary bodies from different serotypes of chlamydia trachomatis and their interaction with mouse fibroblasts
Physicochemical surface properties of elementary bodies from different serotypes of chlamydia trachomatis and their interaction with mouse fibroblasts
复制标题
沙眼衣原体不同血清型原体的理化表面特性及其与小鼠成纤维细胞的相互作用
DOI：
10.1128/iai.36.3.893-899.1982
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发表时间：
1982
影响因子：
3.1
通讯作者：
E. Kihlström
中科院分区：
文献类型：
--
作者：
G. Söderlund;E. Kihlström
采用双水相分配、疏水相互作用层析和离子交换层析等方法研究了雷诺格拉芬纯化的E型和L1型沙眼衣原体基本体的表面性质。这两种血清型对疏水相互作用和表面负电荷的易感性不同。此外，这些参数在两种血清型之间的相互相对大小也不同，这取决于所使用的层析技术。这表明这些层析技术记录了衣原体表面电荷和疏水性的不同方面。DEAE-葡聚糖和葡聚糖硫酸盐影响沙眼衣原体在小鼠成纤维细胞(McCoy细胞)中的结合、穿透和细胞内发育。DEAE-葡聚糖主要通过电荷依赖力影响E型沙眼衣原体与McCoy细胞的结合，而DEAE-葡聚糖和硫酸葡聚糖均主要通过电荷非依赖力影响L1型沙眼衣原体与McCoy细胞的结合。这些结果表明，沙眼衣原体淋巴肉芽肿和非淋巴肉芽肿沙眼衣原体的许多生物学差异可能归因于两株之间的表面特性的差异。
Aqueous biphasic partitioning, hydrophobic interaction chromatography, and ion-exchange chromatography were used to characterize the surface properties of Renografin-purified elementary bodies of Chlamydia trachomatis serotypes E and L1. The two serotypes differed with respect to liability to hydrophobic interaction and negative surface charge. Furthermore, the mutual relative magnitude of these parameters differed between the two serotypes, depending on the chromatographic technique used. This indicates that these chromatographic techniques register different aspects of charge and hydrophobicity on the chlamydial surface. DEAE-dextran and dextran sulfate affected association of, penetration, and intracellular development of C. trachomatis in mouse fibroblasts (McCoy cells). DEAE-dextran affected the association of C. trachomatis serotype E with McCoy cells mainly by charge-dependent forces, whereas both DEAE-dextran and dextran sulfate influenced the association of C. trachomatis serotype L1 mainly by charge-independent forces. These results indicate that the numerous biological differences between lymphogranuloma venereum and non-lymphogranuloma venereum strains of C. trachomatis may be assigned to differences in surface properties between the two strains.