Effect of Wuziyanzong Pill on sperm quality and calcium ion content in oligoasthenospermia rats
Effect of Wuziyanzong Pill on sperm quality and calcium ion content in oligoasthenospermia rats
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五子衍宗丸对少弱精子症大鼠精子质量及钙离子含量的影响
DOI：
10.1016/s0254-6272(13)60083-7
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发表时间：
2012-12-15
影响因子：
2.6
通讯作者：
Li, Jun
中科院分区：
文献类型：
--
作者：
Wang, Tongsheng;Huang, Jinling;Li, Jun
目的：研究五子衍宗对少弱精子症大鼠精子质量和钙离子含量的影响。方法：采用雷公藤多苷30 mg/(kg·d)灌胃，连续8周，建立少弱精子症大鼠模型。随机分为模型组、阳性组(黄精赞育胶囊3.01g/kg)、五子衍宗低、中、高剂量组(生药2.30，4.60，9.20g/kg)，每组10只。另取10只大鼠作为对照组。对照组和模型组大鼠灌胃蒸馏水，其余各组大鼠灌胃五子衍宗30d，摘除附睾体，回收精子，测定精子密度和存活率。结果：雷公藤多苷治疗8周后，模型大鼠精子密度、精子活力和精子钙含量均显著低于对照组(P均<0.05)。
OBJECTIVE: To study the effect of Wuziyanzong treatment on the sperm quality and content of calcium ions (Ca2+) in oligoasthenospermia rats.METHODS: A model of oligoasthenospermia was induced in 50 Sprague Dawley rats by treatment with tripterygium glycosides at 30 mg/kg per day for 8 weeks. They were divided randomly into a model group, a positive group (Huangjingzanyu capsule, 3.01 g/kg), and low, medium and high dose Wuziyanzong treatment groups (2.30, 4.60, 9.20 g/kg crude drug respectively) with 10 in each group. Another 10 rats were used as a control group. The rats in the control and model groups were administered distilled water, while the rats in the remaining groups were administered Wuziyanzong for 30 d. The epididymides were removed, spermatozoa recovered and the sperm density and viability were measured. The spermatozoa were purified and the contents of Ca2+ in the cytoplasm and mitochondria were detected by flow cytometry and atomic absorption spectrometry, respectively.RESULTS: After 8 weeks of treatment with tripterygium glycosides, the sperm density, sperm activity and the Ca2+ content of spermatozoa in the model rats were all significantly decreased compared with the control group (all P