Binding sites for epidermal growth factor in human uterine tissues and leiomyomas.
Binding sites for epidermal growth factor in human uterine tissues and leiomyomas.
复制标题
人类子宫组织和平滑肌瘤中表皮生长因子的结合位点。
DOI：
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发表时间：
1984
影响因子：
5.8
通讯作者：
J. Sanfilippo
中科院分区：
文献类型：
--
作者：
G. Hofmann;C. Rao;G. Barrows;G. Schultz;J. Sanfilippo
There is no published data regarding whether human uterine tissues and leiomyomas contain binding sites for epidermal growth factor (EGF). The present study was undertaken with 33 myometria, 13 leiomyomas, and 4 endometria. All myometria (fundus) and leiomyomas and 3 of 4 endometria specifically bound 125I-labeled mouse EGF (myometria: mean, 1.1; range, 0.1-3.9 fmol/mg protein; leiomyomas: mean, 1.1; range, 0.2-2.6 fmol/mg protein; endometria: mean, 1.0; range, 0.0-3.1 fmol/mg protein). [125I]EGF binding to myometrium was ligand specific in that only unlabeled EGF, but not unlabeled insulin, hCG, human PRL, prostaglandin E1, or prostaglandin F2 alpha, competed with [125I]EGF for binding. The apparent dissociation constants and specific binding capacities for myometrium and leiomyoma were: 0.7 nM, 1.9 fmol/mg protein; and 0.1 and 3.7 nM, 0.1 and 4.4 fmol/mg protein, respectively. While smooth muscle content decreased from the fundus to the cervical end of uteri, [125I]EGF binding did not correspondingly decrease (r = 0.5; n = 4). The binding of [125I]EGF to myometrium did not vary with the phase of the menstrual cycle or the patients' diagnosis before hysterectomy (P greater than 0.1). In summary, these results demonstrate that human uteri and leiomyomas contain specific, high affinity binding sites for EGF, but the binding neither exhibited topographical changes nor varied with the phase of the menstrual cycle or benign pathological state of the tissue.
培养的大鼠颗粒细胞中表皮生长因子对卵巢孕激素产生的调节。
DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Jones,PB;WelshJr,TH;Hsueh,AJ
通讯作者：
Hsueh,AJ
采用高效液相色谱法进一步纯化表皮生长因子。
影响因子：
2.9
作者：
Matrisian,LM;Larsen,BR;Finch,JS;Magun,BE
通讯作者：
Magun,BE