Hypergravity stimulates collagen synthesis in human osteoblast-like cells:: Evidence for the involvement of p44/42 MAP-kinases (ERK 1/2)
Hypergravity stimulates collagen synthesis in human osteoblast-like cells:: Evidence for the involvement of p44/42 MAP-kinases (ERK 1/2)
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DOI：
10.1093/oxfordjournals.jbchem.a022502
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发表时间：
1999-10-01
影响因子：
2.7
通讯作者：
Bätge, B
中科院分区：
文献类型：
--
作者：
Gebken, J;Lüders, B;Bätge, B
骨骼组织的形成和组织受到机械刺激的强烈影响。越来越多的证据表明，重力应力对哺乳动物细胞中早期反应基因的表达有影响，并且可能在细胞外基质的形成中发挥作用。特别是，成骨细胞对重力刺激的反应可能是独特的，因为据报道，在这些细胞中，微重力会减少胶原蛋白的合成，而在成纤维细胞中，观察到相反的效果。在这里，我们研究了离心诱导的超重力对人成骨细胞样细胞（hOB）胶原蛋白合成的影响，并研究了丝裂原激活蛋白（MAP）激酶信号级联的可能参与。在 13 x g 的超重力条件下，胶原蛋白合成显着增加 42 +/- 16%，与 I 型胶原蛋白 α 2 (COL1A2) mRNA 表达增强相平行，hOB 合成的 I、III 和 V 型胶原蛋白比例没有差异，超重力诱导 p44/42 MAP 激酶 (ERK 1/2) 的磷酸化显着升高。抑制该通路可将超重力诱导的胶原合成和 COL1A2 mRNA 表达的刺激抑制约 50%。我们的结果表明，非转化 hOB 的胶原蛋白合成在超重力条件下受到刺激。这种反应似乎部分由 MAP 激酶途径介导。
The formation and organization of skeletal tissue is strongly influenced by mechanical stimulation. There is increasing evidence that gravitational stress has an impact on the expression of early response genes in mammalian cells and may play a role in the formation of extracellular matrix, In particular, osteoblasts may be unique in their response to gravitational stimuli since in these cells microgravity has been reported to reduce collagen synthesis, while in fibroblasts the opposite effect was observed. Here, we hare investigated the influence of hypergravity induced by centrifugation on the collagen synthesis of human osteoblast-like cells (hOB) and studied the possible involvement of the mitogen-activated protein (MAP) kinase signaling cascade. Collagen synthesis was significantly increased by 42 +/- 16% under hypergravity at 13 x g, an effect paralleled by the enhanced expression of the collagen I alpha 2 (COL1A2) mRNA, No difference was seen in the proportion of collagen types I, III, and V synthesized by hOB, Hypergravity induced a markedly elevated phosphorylation of the p44/42 MAP kinases (ERK 1/2). The inhibition of this pathway suppressed the hypergravity-induced stimulation of both collagen synthesis as well as COL1A2 mRNA expression by about 50%. Our results show that the collagen synthesis of non-transformed hOB is stimulated under hypergravitational conditions. This response appears to be partially mediated by the MAP kinase pathway.