Identifying antimicrobial resistance genes of human clinical relevance within Salmonella isolated from food animals in Great Britain
Identifying antimicrobial resistance genes of human clinical relevance within Salmonella isolated from food animals in Great Britain
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DOI：
10.1093/jac/dkq498
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发表时间：
2011-03-01
影响因子：
5.2
通讯作者：
Woodward, Martin J.
中科院分区：
文献类型：
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作者：
Anjum, Muna F.;Choudhary, Suman;Woodward, Martin J.
为了研究英国家畜沙门氏菌中与人类临床相关的耐药基因的发生情况，采用耐药基因芯片和纸片扩散法对225株肠道沙门氏菌分离株进行了鉴定。通过质粒谱分析、接合试验和沙门氏菌基因组岛1（SGI 1）的鉴定，发现约43%的沙门氏菌携带单一或多个耐药基因，其中猪沙门氏菌携带单一或多个耐药基因的比例最高，达96%。将携带多重耐药的分离株分为三组。第1组分离株具有青霉素/链霉素/磺胺/四环素耐药性和相似的表型。该组包含来自猪、牛和家禽的分离株，这些分离株来自几个血清型，包括鼠伤寒4，[5]，12：i：-、德比、俄亥俄州和印第安纳州。所有第2组分离株均来自猪，为鼠伤寒沙门氏菌。它们含有一个非sul型1类整合子和多达13个可转移的抗性。所有第3组分离株均携带1类整合子，并从研究中包括的所有动物种属中分离。从家畜中分离的沙门氏菌对第三代头孢菌素和环丙沙星无耐药性，但携带有耐药基因。猪中多药耐药鼠伤寒沙门氏菌的优势使得引入控制措施（如改进生物安全性）以确保它们不会通过食物链并限制人类治疗选择变得重要。
To investigate the occurrence of antimicrobial resistance genes of human clinical relevance in Salmonella isolated from livestock in Great Britain.Two hundred and twenty-five Salmonella enterica isolates were characterized using an antimicrobial resistance gene chip and disc diffusion assays. Plasmid profiling, conjugation experiments and identification of Salmonella genomic island 1 (SGI1) were performed for selected isolates.Approximately 43% of Salmonella harboured single or multiple antimicrobial resistance genes with pig isolates showing the highest numbers where 96% of Salmonella Typhimurium harboured one or more resistance genes. Isolates harbouring multiple resistances divided into three groups. Group 1 isolates harboured ampicillin/streptomycin/sulphonamide/tetracycline resistance and similar phenotypes. This group contained isolates from pigs, cattle and poultry that were from several serovars including Typhimurium, 4,[5],12:i:-, Derby, Ohio and Indiana. All Group 2 isolates were from pigs and were Salmonella Typhimurium. They contained a non-sul-type class 1 integron and up to 13 transferrable resistances. All Group 3 isolates harboured a class 1 integron and were isolated from all animal species included in the study. Most isolates were Salmonella Typhimurium and harboured SGI1.Salmonella isolated from livestock was shown to harbour antimicrobial resistance genes although no or little resistance to third-generation cephalosporins or ciprofloxacin, respectively, was detected. The preponderance in pigs of multidrug-resistant Salmonella Typhimurium makes it important to introduce control measures such as improved biosecurity to ensure that they do not pass through the food chain and limit human therapeutic options.