Precise mapping of recombination breakpoints suggests a common parent of two BC recombinant HIV type 1 strains circulating in china
Precise mapping of recombination breakpoints suggests a common parent of two BC recombinant HIV type 1 strains circulating in china
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DOI：
10.1089/088922202320567879
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发表时间：
2002-10-15
影响因子：
1.5
通讯作者：
Birx, DL
中科院分区：
文献类型：
--
作者：
McCutchan, FE;Carr, JK;Birx, DL
在中国，已经出现了两种不同的BC重组HIV - 1毒株，并开始在静脉吸毒者中传播。这些重组毒株大多为C亚型，带有一些小的B亚型片段。这两种分别被命名为CRF07_BC和CRF08_BC的重组毒株的更多全基因组序列现已可供分析。对包括本文所述的c54、97CN001、98CN009和新毒株CNGL - 179在内的4种CRF07_BC毒株，以及包括97CNGX - 6、97CNGX - 7、97CNGX - 9和98CN006在内的4种CRF08_BC毒株，通过靴带扫描和重组精细定位软件对其重组断点进行了比较。4种CRF07_BC毒株具有相同的重组结构，4种CRF08_BC毒株具有相同但不同的重组结构。这两种CRF共有5个精确的B/C亚型边界，尽管它们之间的其他片段存在差异，这表明它们有一个共同的祖先，其本身是一种BC重组体，分别“回交”到不同的C亚型毒株上。这两种CRF在中国西部从北到南都有广泛分布，并且在患者间保持着较低的多样性。
Two different BC recombinant HIV-1 strains have arisen and begun to circulate among intravenous drug users in China. The recombinants are mostly subtype C with a few small subtype B segments. Additional full-genome sequences of the two recombinants, termed CRF07_BC and CRF08_BC, are now available for analysis. Four CRF07_BC strains, including c54, 97CN001, 98CN009, and a new strain CNGL-179, described here, and four CRF08_BC strains, including 97CNGX-6, 97CNGX-7, 97CNGX-9, and 98CN006, were compared for their recombination breakpoints by bootscanning and software for fine mapping of recombinants. The four CRF07_BC strains shared an identical recombination structure and the four CRF08 BC strains shared an identical, but different, recombination structure. The two CRFs share five precise subtype B/C boundaries, although although other segments differ between them, suggesting that they shared a common ancestor, itself a BC recombinant that separately "back-crossed" onto different subtype C strains. Both CRFs are broadly distributed from north to south in western China and have maintained low interpatient diversity.