A Simple, Low-Cost Platform for Real-Time Isothermal Nucleic Acid Amplification.
A Simple, Low-Cost Platform for Real-Time Isothermal Nucleic Acid Amplification.
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DOI：
10.3390/s150923418
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发表时间：
2015-09-16
期刊：
影响因子：
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通讯作者：
Balachandran W
中科院分区：
文献类型：
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作者：
Craw P;Mackay RE;Naveenathayalan A;Hudson C;Branavan M;Sadiq ST;Balachandran W
微流控技术的进步和等温核酸扩增分析的引入，导致了一系列适用于现场护理和现场使用的核酸扩增检测解决方案。然而，此类分析仪器的小型化并没有取得如此迅速的进展，基于荧光的分析仍然依赖于复杂、笨重和昂贵的光学设备，如荧光显微镜、光电倍增管和灵敏透镜组件。在这项工作中，我们展示了一个强大的，低成本的平台，用于在微流控设备上进行等温核酸扩增。使用容易获得的材料和商业现成的组件，我们展示了使用低成本的光电二极管和运算放大器进行实时荧光检测，而不需要镜头。设备上的温度调节是使用标准印刷电路板制造方法制造的加热器来实现的。这些简便的施工方法允许以与资源贫乏环境的广泛部署兼容的成本进行制造。
Advances in microfluidics and the introduction of isothermal nucleic acid amplification assays have resulted in a range of solutions for nucleic acid amplification tests suited for point of care and field use. However, miniaturisation of instrumentation for such assays has not seen such rapid advances and fluorescence based assays still depend on complex, bulky and expensive optics such as fluorescence microscopes, photomultiplier tubes and sensitive lens assemblies. In this work we demonstrate a robust, low cost platform for isothermal nucleic acid amplification on a microfluidic device. Using easily obtainable materials and commercial off-the-shelf components, we show real time fluorescence detection using a low cost photodiode and operational amplifier without need for lenses. Temperature regulation on the device is achieved using a heater fabricated with standard printed circuit board fabrication methods. These facile construction methods allow fabrications at a cost compatible with widespread deployment to resource poor settings.