Comparative analytical sensitivities of six rapid influenza A antigen detection test kits for detection of influenza A subtypes H1N1H3N2 and H5N1
Comparative analytical sensitivities of six rapid influenza A antigen detection test kits for detection of influenza A subtypes H1N1H3N2 and H5N1
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DOI：
10.1016/j.jcv.2006.11.010
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发表时间：
2007-02-01
影响因子：
8.8
通讯作者：
Peiris, J. S. M.
中科院分区：
文献类型：
--
作者：
Chan, K. H.;Lam, S. Y.;Peiris, J. S. M.
背景：迫切需要快速简便的方法来诊断人甲型H5N1流感。目前，临床上流行的H5N1病毒抗原快速检测试剂盒对H5N1病毒诊断的敏感性较差。研究设计：研究了用于检测甲型流感的六种市售检测试剂盒。使用病毒培养物作为参考方法，测定了两种当代H1N1、两种H3N2和三种H5N1病毒检测的分析灵敏度。结果和结论：每个检测试剂盒检测H5N1病毒亚型的有效性与检测H1N1或H3N2亚型的常规人类病毒一样。然而，抗原检测试剂盒对所有亚型流感病毒的检测限比病毒分离低1000倍以上。因此，据报道，这些抗原检测试剂盒用于诊断H5N1疾病患者的临床灵敏度差不是由于检测禽流感H5N1与人流感病毒相比的灵敏度差异。(C)2006 Elsevier B.V.保留所有权利。
Background: Rapid and simple methods for diagnosing human influenza A (H5N1) disease urgently needed. The limited data so far suggest that the currently, available rapid antigen detection kits have poor clinical sensitivity for diagnosis of human H5N1 disease.Objectives: To compare the analytical sensitivity of six commercially available rapid antigen detection kits for the detection of "human" (subtypes H1N1, H3N2) and "avian" (subtype H5N1) influenza A viruses.Study design: Six commercially available test kits for the detection of influenza A were investigated. Analytic sensitivity for the detection of two contemporary H1N1, two H3N2 and three H5N1 viruses was determined using virus culture as a reference method.Results and conclusions: Each test kit detected the H5N1 virus subtypes as efficiently as they detected conventional human viruses of subtypes H1N1 or H3N2. However, limits of detection of influenza viruses of all subtypes by antigen detection kits were > 1000-fold lower than virus isolation. Thus, the reportedly poor clinical sensitivity of these antigen detection kits for diagnosis of patients with H5N1 disease is not due to a difference of sensitivity for detecting avian influenza H5N1 compared to human influenza viruses. (C) 2006 Elsevier B.V. All rights reserved.