DSC MEASUREMENT OF CELL-SUSPENSIONS DURING SUCCESSIVE FREEZING RUNS - IMPLICATIONS FOR THE MECHANISMS OF INTRACELLULAR ICE FORMATION
DSC MEASUREMENT OF CELL-SUSPENSIONS DURING SUCCESSIVE FREEZING RUNS - IMPLICATIONS FOR THE MECHANISMS OF INTRACELLULAR ICE FORMATION
复制标题
DOI：
10.1006/cryo.1995.1011
复制
发表时间：
1995-04-01
期刊：
影响因子：
2.7
通讯作者：
BRYANT, G
中科院分区：
文献类型：
--
作者：
BRYANT, G
生物细胞在冷冻过程中形成细胞内冰几乎被认为是致命的，并且是高冷却速率下细胞损伤的主要原因。尽管它很重要，但我们对细胞内冰形成（IIF）机制的理解仍然不完整。本文使用差示扫描量热法研究二甲亚砜 (Me(2)SO) 存在下人淋巴细胞中的 IIF。在初始冷却运行中因 IIF 造成的损坏为 40-60% 的情况下，将样品作为多次连续冷却运行的函数进行研究。这使得不仅可以研究经历 IIF 的细胞部分，还可以研究存活的细胞部分。将发生 IIF 的温度和经历 IIF 的电池体积分数作为连续冷却运行的函数进行分析。利用样品中存在的大量细胞（约 10(6)），检查了连续冷却运行对 IIF 敏感性的影响。 (C) 1995 学术出版社
The formation of intracellular ice in biological cells during freezing is considered almost universally lethal and is the major contributor to cell damage at high cooling rates. Despite its importance, our understanding of the mechanisms of intracellular ice formation (IIF) is still incomplete. In this paper differential scanning calorimetry is used to study IIF in human lymphocytes in the presence of dimethyl sulfoxide (Me(2)SO). Under conditions where damage due to IIF on the initial cooling run is 40-60%, the samples are studied as a function of multiple successive cooling runs. This enables the study not only of the cell fraction which undergoes IIF, but also of the fraction which survives. The temperature at which IIF occurs and the fraction of cell volume which undergoes IIF are analyzed as functions of successive cooling runs. Taking advantage of the large number of cells present in the samples (ca. 10(6)), the effect of successive cooling runs on susceptibility to IIF is examined. (C) 1995 Academic Press, Inc.