Improving rice nitrogen-use efficiency by modulating a novel monouniquitination machinery for optimal root plasticity response to nitrogen
Improving rice nitrogen-use efficiency by modulating a novel monouniquitination machinery for optimal root plasticity response to nitrogen
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DOI：
10.1038/s41477-023-01533-7
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发表时间：
2023-10
期刊：
影响因子：
18
通讯作者：
Yunzhi Huang;Zhe Ji;Yujun Tao;Shuxian Wei;Wu Jiao;Yongzhi Fang;Peng Jian;Chengbo Shen
中科院分区：
文献类型：
--
作者：
Yunzhi Huang;Zhe Ji;Yujun Tao;Shuxian Wei;Wu Jiao;Yongzhi Fang;Peng Jian;Chengbo Shen
植物氮素利用效率（NUE）在很大程度上取决于根系对外源氮的吸收能力，而外源氮的有效性和分配又反过来影响根系构型（RSA）的改变。因此，提高氮响应重塑RSA的最佳氮吸收是一个主要目标，开发高NUE作物。在本研究中，我们鉴定了10号染色体上的N-响应RSA调节基因RNR 10（Regulator OF N-Responsive RSA ON Chromosome 10），它编码一个与生长素生物合成负调控因子DNR 1（DNR NITROGEN RESPONSE 1）相互作用的F-box蛋白。有趣的是，RNR 10单倍半胱氨酸化DNR 1并抑制其降解，从而拮抗生长素积累，导致根系对N和硝酸盐（NO3−）吸收的反应降低。因此，调节RNR 10-DNR 1-生长素模块提供了一种新的策略，用于协调期望的RSA和增强的N获取，以用于未来的可持续农业。
Plant nitrogen (N)-use efficiency (NUE) is largely determined by the ability of root to take up external N sources, whose availability and distribution in turn trigger the modification of root system architecture (RSA) for N foraging. Therefore, improving N-responsive reshaping of RSA for optimal N absorption is a major target for developing crops with high NUE. In this study, we identifiedRNR10(REGULATOR OF N-RESPONSIVE RSA ON CHROMOSOME 10) as the causal gene that underlies the significantly different root developmental plasticity in response to changes in N level exhibited by theindica(Xian) andjaponica(Geng) subspecies of rice.RNR10encodes an F-box protein that interacts with a negative regulator of auxin biosynthesis, DNR1 (DULL NITROGEN RESPONSE1). Interestingly, RNR10 monoubiquitinates DNR1 and inhibits its degradation, thus antagonizing auxin accumulation, which results in reduced root responsivity to N and nitrate (NO3−) uptake. Therefore, modulating the RNR10-DNR1-auxin module provides a novel strategy for coordinating a desirable RSA and enhanced N acquisition for future sustainable agriculture.