Design constraints for mobile, high-speed fluorescence brain imaging in awake animals.
Design constraints for mobile, high-speed fluorescence brain imaging in awake animals.
复制标题
清醒动物的移动高速荧光脑成像的设计限制。
DOI：
10.1109/tbcas.2012.2226174
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发表时间：
2012
影响因子：
5.1
通讯作者：
Pieribone,VincentA
中科院分区：
文献类型：
--
作者：
Osman,Ahmad;Park,JoonHyuk;Dickensheets,David;Platisa,Jelena;Culurciello,Eugenio;Pieribone,VincentA
在本文中，我们提出了一个完全独立的成像仪器（总长度30毫米），能够记录高速和检测相对较小的荧光信号（0.1% ΔF/F），这些信号来自可能含有基因编码传感器或染料的脑组织。该装置有可能在清醒和活动动物的自然行为中研究神经元活动，而无需麻醉和约束的压力和抑制。该装置是一个完全独立的照明系统，宽视场荧光显微镜（~ 4.8mm2FOV-25 um横向分辨率-1.8 ×放大-0.39 NA）和CMOS图像传感器（32 × 32）。该系统的总重量为10克，成像速度可达每秒900帧。我们提出了电压染色RH1692实验，利用该系统研究小鼠在胡须运动过程中的体感觉皮层。
In this paper we present a fully self-contained imaging instrument (30 mm overall length) that is capable of recording high speed and detect relatively small fluorescent signals (0.1% ΔF/F) from brain tissues potentially containing genetically-encoded sensors or dyes. This device potentially enables the study of neuronal activity in awake and mobile animals during natural behaviors without the stress and suppression of anesthesia and restraint. The device is a fully self-contained illumination system, wide field fluorescence microscope ( ~ 4.8mm2FOV-25 um lateral resolution-1.8 × magnification-0.39 NA) and CMOS image sensor (32 × 32). The total weight of the system is 10 g and is capable of imaging up to 900 fps. We present voltage dye RH1692 experiments using the system to study the somatosensory cortex of mice during whisker movements using an air puff.