Surface-enhanced Raman spectroscopy as a tool for probing specific biochemical components in bacteria
Surface-enhanced Raman spectroscopy as a tool for probing specific biochemical components in bacteria
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DOI：
10.1366/000370204322729441
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发表时间：
2004-01-01
影响因子：
3.5
通讯作者：
Efrima, S
中科院分区：
文献类型：
--
作者：
Zeiri, L;Bronk, BV;Efrima, S
用银处理细菌会产生来自银胶体附近各种细胞化学成分的强烈且高度特异的表面增强拉曼光谱 (SERS) 光谱。特别是，我们证明了对与细胞包膜相关的黄素成分及其氧化状态极其敏感。从细胞的可溶性内部部分获得不同的光谱，可能与 DNA、羧酸盐和可能的磷酸盐有关。
Treatment of bacteria with silver yields intense and highly specific surface-enhanced Raman spectroscopy (SERS) spectra from various cellular chemical components located in the vicinity of the silver colloids. In particular, we demonstrate an extreme sensitivity to flavin components associated with the cell envelope and to their state of oxidation. Different spectra, possibly associated with DNA, carboxylates, and perhaps phosphates, are obtained from the soluble interior fraction of the cell.