A transcriptional activator, AoXlnR, controls the expression of genes encoding xylanolytic enzymes in Aspergillus oryzae
A transcriptional activator, AoXlnR, controls the expression of genes encoding xylanolytic enzymes in Aspergillus oryzae
复制标题
DOI：
10.1006/fgbi.2001.1321
复制
发表时间：
2002-03-01
影响因子：
3
通讯作者：
Tsukagoshi, N
中科院分区：
文献类型：
--
作者：
Marui, J;Tanaka, A;Tsukagoshi, N
通过在编码主要曲霉木聚糖酶的xynF 1基因的启动子区域上缺失，显示了含有XlnR结合序列GGCTAAA以及两个类似序列的53-bp DNA片段赋予该基因木聚糖诱导。从黑曲霉中克隆了编码尼日尔xInR同源基因（简称AoxInR）的互补DNA和基因组DNA。测序。AoXlnR由971个氨基酸组成，N端为锌双核簇结构域，与A.尼日尔XlnR.包含N-末端部分的锌簇区域的重组AoXlnR蛋白以约低10倍的亲和力结合共有结合序列及其同源序列GGCTGA。GGCTA/GA更适合作为XlnR共有结合序列。这两个序列在体内XlnR介导的xynF 1基因诱导中独立发挥作用。这通过使用AoxInR破坏剂进一步证实。xynF 1和xylA基因在破坏物中均不表达，表明木聚糖诱导基因在A.也可以以与针对A所描述的相同的方式来控制λ。尼日尔. (C)2002 Elsevier Science（美国）。
By deletion across the promoter region of the xynF1 gene encoding the major Aspergillus oryzae xylanase, a 53-bp DNA fragment containing the XlnR binding sequence GGCTAAA as well as two similar sequences was shown to confer xylan inducibility on the gene. Complementary and genomic DNAs encoding the Aspergillus niger xInR homologous gene, abbreviated AoxInR, were cloned from A. oryzae and sequenced. AoXlnR comprised 971 amino acids with a zinc binuclear cluster domain at the N-terminal region and revealed 77.5% identity to the A. niger XlnR. Recombinant AoXlnR protein encompassing the zinc cluster region of the N-terminal part bound to both the consensus binding sequence and its cognate sequence, GGCTGA, with an approximately 10 times lower affinity. GGCTA/GA is more appropriate as the XlnR consensus binding sequence. Both sequences functioned independently in vivo in XlnR-mediating induction of the xynF1 gene. This was further confirmed by using an AoxInR disruptant. Neither the xynF1 nor the xylA gene was expressed in the disruptant, suggesting that the xylan-inducible genes in A. oryzae may also be controlled in the same manner as described for A. niger. (C) 2002 Elsevier Science (USA).