Application of high-throughput, high-resolution and cost-effective next generation sequencing-based large-scale HLA typing in donor registry.
Application of high-throughput, high-resolution and cost-effective next generation sequencing-based large-scale HLA typing in donor registry.
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高通量、高分辨率和经济高效的基于下一代测序的大规模 HLA 分型在捐献者登记中的应用。
DOI：
10.1111/tan.12477
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发表时间：
2015
期刊：
影响因子：
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通讯作者：
W. Wang
中科院分区：
文献类型：
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作者：
M. Zhou;D. Gao;X. Chai;J. Liu;Z. Lan;Q. Liu;F. Yang;Y. Guo;J. Fang;L. Yang;D. Du;L. Chen;X. Yang;M. Zhang;H. Zeng;Jun Lu;H. Chen;X. Zhang;S. Wu;Y. Han;J. Tan;Z. Cheng;C. Huang;W. Wang
采用基于新一代测序（NGS）的人白细胞抗原（HLA）分型技术对中国骨髓捐献计划（CMDP）登记供者进行超大规模基因分型。超过79000份样本在4位等位基因水平上进行了HLA- a、-B、-C、DRB1和DQB1位点的基因分型，没有歧异，在HiSeq流式细胞中每车道的吞吐量高达2068份样本（每趟8车道），与基于sanger的分型相比，成本降低了95%。随机抽取2%的样本由CMDP QC实验室进行4位等位基因水平的质量控制（QC）检测，一致性为99.72%。这些结果表明，NGS是一种具有成本效益和有价值的HLA分型工具。
Next generation sequencing (NGS)-based human leukocyte antigen (HLA) typing was used for ultra large-scale genotyping of registry donors for the China Marrow Donor Program (CMDP). More than 79,000 samples were subjected to HLA genotyping at 4-digit allelic level without ambiguities for HLA-A, -B, -C, DRB1 and DQB1 loci, with throughput up to 2068 samples per lane in a HiSeq flow cell (eight lanes per run), and cost reduced by 95% compared with that of Sanger-based typing. Two percent of randomly selected samples were quality control (QC) tested at 4-digit allelic level by the CMDP QC laboratory, yielded a concordance of 99.72%. These results demonstrate that NGS is a cost effective and valuable tool for HLA typing of registry donors.
影响因子：
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作者：
Bentley G;Higuchi R;Hoglund B;Goodridge D;Sayer D;Trachtenberg EA;Erlich HA
通讯作者：
Erlich HA
影响因子：
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作者：
Holcomb CL;Höglund B;Anderson MW;Blake LA;Böhme I;Egholm M;Ferriola D;Gabriel C;Gelber SE;Goodridge D;Hawbecker S;Klein R;Ladner M;Lind C;Monos D;Pando MJ;Pröll J;Sayer DC;Schmitz-Agheguian G;Simen BB;Thiele B;Trachtenberg EA;Tyan DB;Wassmuth R;White S;Erlich HA
通讯作者：
Erlich HA