Fluid- or surface-phase human salivary scavenger protein gp340 exposes different bacterial recognition properties
Fluid- or surface-phase human salivary scavenger protein gp340 exposes different bacterial recognition properties
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DOI：
10.1128/iai.73.4.2245-2252.2005
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发表时间：
2005-04-01
影响因子：
3.1
通讯作者：
Strömberg, N
中科院分区：
文献类型：
--
作者：
Loimaranta, V;Jakubovics, NS;Strömberg, N
唾液清道夫受体富含半胱氨酸的蛋白gp 340聚集链球菌和其他细菌，作为粘膜表面宿主先天防御系统的一部分。在这篇文章中，我们研究了液相gp 340和羟基磷灰石表面吸附的gp 340在草绿色组链球菌（例如，戈登链球菌（Streptococcus gordonii）和变形链球菌（Streptococcus mutans）），非草绿色链球菌（non-viridans group streptocci）（例如，化脓性链球菌和猪链球菌）和口腔放线菌。流体相gp 340和表面相gp 340生物型被链球菌识别，根据其与gp 340的相互作用模式形成三个表型分组。组I链球菌聚集和粘附到gp 340，和组II链球菌优先粘附到表面结合的gp 340，而组III链球菌优先聚集的gp 340。发现测试的每种链球菌都含有代表这些gp 340相互作用分组中至少两种的菌株。gp 340相互作用模式I至III和糖特异性的gp 340结合菌株相吻合的几个物种。许多gp 340相互作用是唾液酸酶敏感的，并且S. Gordonii与唾液酸特异性的变体相关。坚持S。戈登氏菌DL 1（查利斯）与表面结合的gp 340的结合依赖于唾液酸结合粘附素Hsa的表达。然而，通过液相gp 340的细胞聚集独立于Hsa，并且涉及SspA和SspB（抗原I/II家族）多肽。相反，gp 340介导的S.变异株NG 8涉及抗原I/II多肽。在S.化脓性链球菌导致通过GP 340的细胞聚集增加。这些结果表明，唾液gp 340识别不同的细菌受体，根据gp 340是否存在于流体相或表面结合。不同毒力水平和不同来源的链球菌种的gp 340的这种阶段相关的差异识别可能介导替代宿主对寄生菌或致病性细菌表型的反应。
Salivary scavenger receptor cysteine-rich protein gp340 aggregates streptococci and other bacteria as part of the host innate defense system at mucosal surfaces. In this article, we have investigated the properties of fluid-phase gp340 and hydroxylapatite surface-adsorbed gp340 in aggregation and adherence, respectively, of viridans group streptococci (e.g., Streptococcus gordonii and Streptococcus mutans), non-viridans group streptococci (e.g., Streptococcus pyogenes and Streptococcus suis), and oral Actinomyces. Fluid-phase gp340 and surface-phase gp340 bioforms were differentially recognized by streptococci, which formed three phenotypic groupings according to their modes of interaction with gp340. Group I streptococci were aggregated by and adhered to gp340, and group II streptococci preferentially adhered to surface-bound gp340, while group III streptococci were preferentially aggregated by gp340. Each species of Streptococcus tested was found to contain strains representative of at least two of these gp340 interaction groupings. The gp340 interaction modes I to III and sugar specificities of gp340 binding strains coincided for several species. Many gp340 interactions were sialidase sensitive, and each of the interaction modes (I to III) for S. gordonii was correlated with a variant of sialic acid specificity. Adherence of S. gordonii DL1 (Challis) to surface-bound gp340 was dependent upon expression of the sialic acid binding adhesin Hsa. However, aggregation of cells by fluid-phase gp340 was independent of Hsa and involved SspA and SspB (antigen I/II family) polypeptides. Conversely, both gp340-mediated aggregation and adherence of S. mutans NG8 involved antigen I/II polypeptide. Deletion of the mga virulence regulator gene in S. pyogenes resulted in increased cell aggregation by gp340. These results suggest that salivary gp340 recognizes different bacterial receptors according to whether gp340 is present in the fluid phase or surface bound. This phase-associated differential recognition by gp340 of streptococcal species of different levels of virulence and diverse origins may mediate alternative host responses to commensal or pathogenic bacterial phenotypes.