Lactotrophs secreting small amounts of prolactin reveal great responsiveness to thyrotropin-releasing hormone: analysis by the sequential cell immunoblot assay.
Lactotrophs secreting small amounts of prolactin reveal great responsiveness to thyrotropin-releasing hormone: analysis by the sequential cell immunoblot assay.
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分泌少量催乳素的泌乳素显示出对促甲状腺素释放激素的强烈反应：通过序贯细胞免疫印迹测定进行分析。
DOI：
10.1210/endo.130.6.1597137
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发表时间：
1992
期刊：
影响因子：
4.8
通讯作者：
F. Kimura
中科院分区：
文献类型：
--
作者：
J. Arita;Y. Kojima;F. Kimura
用序贯细胞免疫印迹法研究了TRH对雌性大鼠单个催乳细胞PRL分泌的影响。首先孵育培养在盖玻片上的相同垂体细胞以确定PRL的基础分泌，随后用不同浓度的TRH之一进行挑战。从单个催乳素分泌的PRL被吸收在蛋白质印迹转移膜上，免疫染色，并通过显微图像分析进行定量。当没有TRH添加到第二次孵育（对照）中使用的培养基中时，从单个催乳素分泌的PRL的量是第一次孵育中分泌的量的93%。在第二次孵育中用3 × 10（-10）-10（-7）M TRH处理，以剂量依赖性方式增加催乳素分泌显著高于对照组中催乳素分泌置信限的催乳素分泌细胞的比例。然而，即使在TRH的最大有效浓度下，TRH反应性催乳细胞的百分比仍低于50%。TRH-反应性催乳素的比例显着更大的细胞分泌少量的催乳素在基础条件下比那些分泌大量。此外，小而不是大的分泌细胞显示出10（-7）M TRH分泌的PRL的平均绝对量显著增加，其代表不少于TRH处理从催乳细胞群体分泌的所有PRL的45%。这些结果表明，1）催乳素对TRH的反应性存在异质性，2）在基础条件下分泌少量PRL的催乳素群体比分泌大量PRL的催乳素群体含有更大比例的TRH反应性催乳素。
The effects of TRH on PRL secretion from individual lactotrophs of female rats were investigated by using a sequential cell immunoblot assay. The same pituitary cells cultured on coverslips were first incubated to determine basal secretion of PRL and subsequently challenged by one of various concentrations of TRH. The PRL secreted from the single lactotrophs was absorbed on protein-blotting transfer membranes, immunostained, and quantified by microscopic image analysis. When no TRH was added to the medium used in the second incubation (controls), the amount of PRL secreted from individual lactotrophs was 93% of that secreted in the first incubation. Treatment with 3 x 10(-10)-10(-7) M TRH in the second incubation increased in a dose-dependent manner the proportion of lactotrophs whose PRL secretion was significantly greater than confidence limits for PRL secretion in the controls. However, the percentage of TRH-responsive lactotrophs remained less than 50% even at a maximally effective concentration of TRH. Proportions of the TRH-responsive lactotrophs were significantly greater in cells that secreted small amounts of PRL under basal conditions than in those that secreted large amounts. Furthermore, the small, but not the large, secretors showed a significant increase in mean absolute amounts of PRL secreted by 10(-7) M TRH, which represented no less than 45% of all PRL secreted from the lactotroph population by the TRH treatment. These results indicate that 1) there is a heterogeneity with respect to lactotroph responsiveness to TRH and that 2) a population of lactotrophs that secrete small amounts of PRL under basal conditions contains a much larger proportion of TRH-responsive lactotrophs than does a population of lactotrophs that secrete large amounts of PRL.
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促甲状腺激素释放激素诱导垂体细胞中胞质游离 Ca2 浓度的峰值和平台。
DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Albert,PR;TashjianJr,AH
通讯作者：
TashjianJr,AH
新合成的催乳素颗粒的优先释放是促乳腺激素细胞功能异质性的结果。
影响因子：
4.8
作者：
Walker,AM;Farquhar,MG
通讯作者：
Farquhar,MG
细胞印迹：一种量化单个大鼠垂体细胞激素分泌的新方法。
影响因子：
4.8
作者：
Kendall,ME;Hymer,WC
通讯作者：
Hymer,WC
哺乳会增加对各种催乳素释放刺激作出反应的催乳激素的比例。
影响因子：
4.8
作者：
Nagy,GM;Frawley,LS
通讯作者：
Frawley,LS
测量同一细胞中的肽分泌和基因表达。
DOI：
10.1210/mend-5-1-134
发表时间：
1991
期刊：
Molecular endocrinology (Baltimore, Md.)
影响因子：
--
作者：
Scarbrough,K;Weiland,NG;Larson,GH;Sortino,MA;Chiu,SF;Hirshfield,AN;Wise,PM
通讯作者：
Wise,PM