Consolidated production of coniferol and other high-value aromatic alcohols directly from lignocellulosic biomass
Consolidated production of coniferol and other high-value aromatic alcohols directly from lignocellulosic biomass
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DOI：
10.1039/c9gc02359c
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发表时间：
2020-01-07
期刊：
影响因子：
9.8
通讯作者：
Dixon, Neil
中科院分区：
文献类型：
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作者：
Tramontina, Robson;Galman, James L.;Dixon, Neil
利用可再生生物质可持续地生产精细化学品和生物燃料，为继续利用有限的石油地质储量提供了一种潜在的替代办法。然而，为了与当前的石化精炼工艺竞争，替代生物精炼工艺必须克服显著的成本和生产力障碍。在此，我们首次展示了直接从木质纤维素生物质中生物催化生产多功能化学结构单元松柏醇。在用阿魏酸酯酶（XynZ）、羧酸还原酶（CAR）和醛酮还原酶（AKR）对木质纤维素进行生物催化处理以释放和转化阿魏酸之后，这种全细胞催化级联不仅实现了与碱水解相比从木质纤维素中相当的阿魏酸释放，而且还显示出阿魏酸向松柏醇的有效转化。该系统代表了从废弃植物生物质生产高价值精细化学品的综合生物降解-生物转化策略，具有最大限度减少环境废物和增加农工业残留物价值的潜力。
Sustainable production of fine chemicals and biofuels from renewable biomass offers a potential alternative to the continued use of finite geological oil reserves. However, in order to compete with current petrochemical refinery processes, alternative biorefinery processes must overcome significant costs and productivity barriers. Herein, we demonstrate the biocatalytic production of the versatile chemical building block, coniferol, for the first time, directly from lignocellulosic biomass. Following the biocatalytic treatment of lignocellulose to release and convert ferulic acid with feruloyl esterase (XynZ), carboxylic acid reductase (CAR) and aldo-keto reductase (AKR), this whole cell catalytic cascade not only achieved equivalent release of ferulic acid from lignocellulose compared to alkaline hydrolysis, but also displayed efficient conversion of ferulic acid to coniferol. This system represents a consolidated biodegradation-biotransformation strategy for the production of high value fine chemicals from waste plant biomass, offering the potential to minimize environmental waste and add value to agro-industrial residues.