DEPOLARIZATION-INDUCED RELEASE OF CORTICOTROPIN-RELEASING FACTOR (CRF) IN PRIMARY NEURONAL CULTURES OF THE AMYGDALA
DEPOLARIZATION-INDUCED RELEASE OF CORTICOTROPIN-RELEASING FACTOR (CRF) IN PRIMARY NEURONAL CULTURES OF THE AMYGDALA
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DOI：
10.1016/0143-4179(94)90102-3
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发表时间：
1994-02-01
期刊：
影响因子：
2.9
通讯作者：
BIRKLE, DL
中科院分区：
文献类型：
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作者：
CRATTY, MS;BIRKLE, DL
促肾上腺皮质激素释放因子 (CRF) 是一种由 41 个氨基酸组成的神经肽，分布在整个中枢神经系统中，似乎在压力、焦虑和抑郁中发挥着关键作用。 CRF 在大脑边缘区域杏仁核中浓度很高，杏仁核是对压力的情绪和自主反应的重要区域。在本报告中，使用胎鼠大脑 (E18-E19) 的杏仁核原代神经元培养物来研究去极化诱导的 CRF 释放。对培养物的免疫细胞化学分析显示，约 1% 的神经元中存在 CRF 免疫反应性 (CRFir) 的珠状分布。时程研究表明，56 mM KCI 诱发的 CRF 释放在第一分钟内发生初始爆发，并维持了 30 分钟以上；基础 CRF 释放在 30 分钟内略有增加。响应去极化的 CRF 释放随着细胞密度的增加和培养天数的增加而增加。多次连续孵育交替基础和去极化条件导致可释放的 CRF 池耗尽。钾诱发的 CRF 释放是钙依赖性的。这些数据表明，胎鼠杏仁核的原代神经元培养物是研究该大脑区域 CRF 释放的有效模型系统。
The 41-amino acid neuropeptide, corticotropin-releasing factor (CRF) is distributed throughout the central nervous system and appears to play a pivotal role in stress, anxiety and depression. CRF is present in high concentrations in the limbic brain region, the amygdala, an area important in emotional and autonomic responses to stress. In this report, primary neuronal cultures of amygdala from fetal rat brains (E18-E19) were used to study depolarization-induced CRF release. Immunocytochemical analyses of the cultures revealed a bead-like distribution of CRF immunoreactivity (CRFir) in about 1% of the neurons. Time course studies showed that 56 mM KCI-evoked CRF release occurred with an initial burst during the first minute that was maintained over 30 min; basal CRF release slightly increased over a 30-min period. CRF release in response to depolarization increased with increasing cell density and with increasing days in culture. Multiple serial incubations alternating basal and depolarizing conditions caused a depletion of the releasable pool of CRF. Potassium-evoked CRF release was calcium-dependent. These data suggest that primary neuronal cultures of fetal rat amygdala are an effective model system to study CRF release in this brain region.