Prevalence and clinical features of human papillomavirus in head and neck squamous cell carcinoma in Okinawa, southern Japan
Prevalence and clinical features of human papillomavirus in head and neck squamous cell carcinoma in Okinawa, southern Japan
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DOI：
10.1007/s00405-011-1515-0
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发表时间：
2011-11-01
影响因子：
2.6
通讯作者：
Suzuki, Mikio
中科院分区：
文献类型：
--
作者：
Deng, Zeyi;Hasegawa, Masahiro;Suzuki, Mikio
日本南部亚热带岛屿冲绳岛此前的研究表明，与日本本土相比，口腔癌中人乳头瘤病毒（HPV）的患病率较高，口腔癌和咽癌的发病率也较高。本研究旨在调查冲绳地区头颈鳞状细胞癌 (HNSCC) 中 HPV 的流行病学和临床特征。使用三个共有引物组通过 PCR 筛查 150 名冲绳头颈鳞状细胞癌 (HNSCC) 患者的总共 150 个 DNA 样本中的 HPV 序列，并通过直接测序确定 HPV 类型。其中喉咽部标本46例，口咽部标本44例，喉部标本16例，口腔标本25例，上颌窦标本10例，鼻咽部标本9例。在 45 例 (30.0%) HNSCC 中检测到 HPV DNA，在 86.7% 的阳性标本中检测到 HPV-16。 HPV序列的患病率最高的是口咽癌（50.0%），尤其是扁桃体癌（63.6%）。多变量分析显示，口咽癌（P = 0.002；OR = 5.34；95% CI = 1.83-15.58）、口腔癌（P = 0.012；OR = 4.94；95% CI = 1.43-17.10）和组织学低分化（P = 0.011；OR = 4.25；P = 0.011；OR = 4.25）。 95% CI = 1.39-13.04) 各自独立地增加 HPV 感染的患病率。本研究显示，与日本本土相比，冲绳的 HNSCC（例如口咽癌和口腔癌）患者的 HPV-16 阳性率相对较高，而 HPV-18 阳性率较低。
Previous studies from Okinawa, a subtropical island in southern Japan, demonstrated a higher prevalence of human papillomavirus (HPV) in oral carcinoma and a higher incidence of oral and pharyngeal carcinoma than those for mainland Japan. The present study aims to investigate epidemiologic and clinical features of HPV in head and neck squamous cell carcinoma (HNSCC) in Okinawa. A total of 150 DNA samples from 150 Okinawan patients with head and neck squamous cell carcinoma (HNSCC) were screened for HPV sequences by PCR using three consensus primer sets, and HPV types were determined by direct sequencing. The samples were consisted of 46 cases from the hypopharynx, 44 from the oropharynx, 16 from the larynx, 25 from the oral cavity, 10 from the maxillary sinus, and 9 from the nasopharynx. HPV DNA was detected in 45 (30.0%) HNSCCs, and HPV-16 was identified in 86.7% of positive specimens. The highest prevalence of the HPV sequence was found in oropharyngeal carcinomas (50.0%), especially in tonsillar cancer (63.6%). Multivariate analysis showed that oropharyngeal carcinoma (P = 0.002; OR = 5.34; 95% CI = 1.83-15.58), oral cavity carcinoma (P = 0.012; OR = 4.94; 95% CI = 1.43-17.10), and histological poor differentiation (P = 0.011; OR = 4.25; 95% CI = 1.39-13.04) each independently increased the prevalence of HPV infection. The present study reveals that patients with HNSCC, e.g., oropharyngeal and oral cavity carcinomas, in Okinawa have relatively high HPV-16 positive rates and low HPV-18 positive rates comparing with mainland Japan.