In vivo evaluation of poly(L-lactic acid) porous conduits for peripheral nerve regeneration
In vivo evaluation of poly(L-lactic acid) porous conduits for peripheral nerve regeneration
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DOI：
10.1016/s0142-9612(99)00010-1
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发表时间：
1999-06-01
期刊：
影响因子：
14
通讯作者：
Patrick, CW
中科院分区：
文献类型：
--
作者：
Evans, GRD;Brandt, K;Patrick, CW
本研究利用大鼠10 mm坐骨神经缺损模型，对聚乳酸-L(PLLA)作为一种可生物降解的多孔神经导管进行体内实验。PLLA导管由挤压技术制成，内径为1.6 mm，外径为3.2 mm，长度为12 mm。它们具有高度的渗透性和相互连通的孔结构(孔隙率为83.5%，平均孔径为12.1微米)。采用显微外科技术将导管植入SD大鼠右侧坐骨神经缺损处，以同种异体神经移植为对照。术后每月至16周进行步行轨迹分析。在6周和16周结束时，从同种异体移植物/管道和远端神经切取切片进行组织形态计量学分析。取右侧腓肠肌，测定其重量。所有管道都完好无损，没有破损。此外，在放置的16周期间，没有导管延长。步行轨迹分析和腓肠肌重量显示，在16周的时间里，管道和同种异体对照组的腓肠肌再生都在增加，而控制值明显更大。16周时，PLLA管道远端坐骨神经的神经纤维密度(0.16+/-0.07)与对照组(0.19+/-0.05)相近。16周时PLLA管道远端坐骨神经轴突数/mm(2)低于同种异体神经(13800+/-2500和10700+/-4700)。PLLA的结果比先前研究的75：25聚(DL-乳酸-羟基乙酸)明显改善，提示PLLA多孔管可能作为周围神经再生的支架。(C)1999爱思唯尔科学有限公司。保留所有权利。
The present study provides in vivo trials of poly(L-lactic acid) (PLLA) as a porous biodegradable nerve conduit using a 10 mm sciatic nerve defect model in rats. The PLLA conduits, fabricated by an extrusion technique, had an inner diameter of 1.6 mm, an outer diameter of 3.2 mm, and a length of 12 mm. They were highly porous with an interconnected pore structure (of 83.5% porosity and 12.1 mu m mean pore size). The conduits were interposed into the right sciatic nerve defect of Sprague Dawley rats using microsurgical techniques; nerve isografts served as controls. Walking track analysis was performed after conduit placement monthly through 16 weeks. At the conclusion of 6 and 16 weeks, sections from the isograft/conduit and distal nerve were harvested for histomorphometric analysis. The right gastrocnemius muscle was also harvested and its weight was determined. All conduits remained intact without breakage. Moreover, no conduit elongated during the 16 weeks of placement. Walking track analysis and gastrocnemius muscle weight demonstrated increasing regeneration over the 16 weeks in both the conduit and isograft control groups, with control values significantly greater. The nerve fiber density in the distal sciatic nerve for the PLLA conduits (0.16 +/- 0.07) was similar to that for the control isografts (0.19 +/- 0.05) at 16 weeks. The number of axons/mm(2) in the distal sciatic nerve for the PLLA conduits was lower than that for the isografts (13 800 +/- 2500 vs. 10 700 +/- 4700) at 16 weeks. The results for PLLA were significantly improved over those for 75:25 poly(DL-lactic-co-glycolic acid) of a previous study and suggest that PLLA porous conduits may serve as a scaffold for peripheral nerve regeneration. (C) 1999 Elsevier Science Ltd. All rights reserved.