Treatment of microbiologically polluted aquaculture waters by a novel photochemical technique of potentially low environmental impact
Treatment of microbiologically polluted aquaculture waters by a novel photochemical technique of potentially low environmental impact
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DOI：
10.1039/b606975d
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发表时间：
2006-01-01
影响因子：
--
通讯作者：
Jori, Giulio
中科院分区：
文献类型：
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作者：
Magaraggia, Michela;Faccenda, Filippo;Jori, Giulio
基于可见光（包括太阳光）和卟啉型光敏剂的联合作用，研究了一种新的方法对养鱼池微生物污染的沃茨进行消毒的适用性，该方法使用（a）革兰氏阳性细菌（耐甲氧西林金黄色葡萄球菌）、革兰氏阴性细菌（大肠杆菌）和真菌病原体（水霉属）的细胞培养物; (b)试验性水产养殖厂，包括自发或人工感染水霉的虹鳟鱼（Oncorhynchus mykiss）。通过使用两种阳离子卟啉，即四取代的N-甲基-吡啶基卟啉（C1）和其中一个N-甲基被N-十四烷基链取代的其类似物（C14），和低强度可见光照射显示了广泛的（最多6 - 7 log）减少细菌/在微摩尔浓度的光敏剂存在下，在短的孵育和照射时间后的真菌种群。此外，C14在没有光的情况下也显示出一定的毒性作用。扩展这些研究的试点工厂表明，C1 +光和C14可以防止水霉感染或促进治愈感染的鳟鱼水霉病的治疗与亚微摩尔卟啉剂量。该程序似乎成本低，对环境影响小。
The applicability of a novel procedure for the disinfection of microbiologically polluted waters from fish-farming ponds, based on the combined action of visible light (including sunlight) and porphyrin- type photosensitising agents, has been investigated using (a) cell cultures of a Gram-positive bacterium (meticillin-resistant Staphylococcus aureus), a Gram-negative bacterium (Escherichia coli) and a fungal pathogen (Saprolegnia spp.); (b) pilot aquaculture plants involving either spontaneously or artificially Saprolegnia-infected rainbow trout (Oncorhynchus mykiss). The results obtained by using two cationic porphyrins, namely a tetra-substituted N-methyl-pyridylporphine (C1) and its analogue where one N-methyl group had been replaced by a N-tetradecyl chain (C14), and low intensity visible light irradiation showed an extensive (up to 6 - 7 log) decrease in the bacterial/ fungal population after short incubation and irradiation times in the presence of micromolar photosensitiser concentrations. Moreover, C14 showed some toxic effect also in the absence of light. Extension of these studies to the pilot plants indicated that both C1 + light and C14 can prevent Saprolegnia infections or promote the cure of saprolegniasis in infected trout by treatments with submicromolar porphyrin doses. The procedure appears to be of low cost and to have a low environmental impact.