Requirement for eukaryotic translation initiation factors in cap-independent translation differs between bipartite genomic RNAs of red clover necrotic mosaic virus.
Requirement for eukaryotic translation initiation factors in cap-independent translation differs between bipartite genomic RNAs of red clover necrotic mosaic virus.
复制标题
红三叶草坏死花叶病毒的二分基因组 RNA 在帽独立翻译中对真核翻译起始因子的要求不同。
DOI：
10.1016/j.virol.2017.06.015
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Okuno T.
中科院分区：
文献类型：
--
作者：
Tajima Y;Iwakawa HO;Hyodo K;Kaido M;Mise K;Okuno T.
红三叶坏死性花叶病毒(RCNMV)的两部分基因组RNA没有5‘帽和3’聚(A)尾。RNA1编码病毒复制蛋白，RNA2编码运动蛋白(MP)。这些蛋白质以帽不依赖的方式翻译。我们先前鉴定了两个协同作用的RNA元件，它们协同地将真核细胞翻译起始因子(EIF)复合体eIF4F或eIFso4F招募到RNA1。在RNA2中还没有发现Suchcis作用的RNA元件和宿主因子。在这里，我们发现RNA1的翻译在拟南芥中受到了显著的损害。RNA1在iifso4F1突变体中高效复制，表明RNA1的复制蛋白在植物中得到了旺盛的翻译。相反，4f1突变株的MP积累减少，而4f1突变株的MP积累没有减少。综上所述，这些结果表明RCNMV使用不同的EIF复合体来翻译其两部分基因组RNA，这可能有助于微调病毒感染期间的基因表达。
The bipartite genomic RNAs of red clover necrotic mosaic virus (RCNMV) lack a 5′ cap and a 3′ poly(A) tail. RNA1 encodes viral replication proteins, and RNA2 encodes a movement protein (MP). These proteins are translated in a cap-independent manner. We previously identified twocis-acting RNA elements that cooperatively recruit eukaryotic translation initiation factor (eIF) complex eIF4F or eIFiso4F to RNA1. Suchcis-acting RNA elements and host factors have not been identified in RNA2. Here we found that translation of RNA1 was significantly compromised inArabidopsis thalianacarryingeif4fmutation. RNA1 replicated efficiently ineifiso4f1mutants, suggesting vigorous translation of the replication proteins from RNA1 in the plants. In contrast, MP accumulation was decreased ineifiso4f1mutants but not ineif4fmutants. Collectively, these results suggest that RCNMV uses different eIF complexes for translation of its bipartite genomic RNAs, which may contribute to fine-tuning viral gene expression during infection.