Near infrared spectra indicate specific mutant endosperm genes and reveal a new mechanism for substituting starch with (1→3,1→4)-β-glucan in barley
Near infrared spectra indicate specific mutant endosperm genes and reveal a new mechanism for substituting starch with (1→3,1→4)-β-glucan in barley
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DOI：
10.1016/j.jcs.2004.07.006
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发表时间：
2004-11-01
影响因子：
3.8
通讯作者：
Sondergaard, I
中科院分区：
文献类型：
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作者：
Munck, L;Moller, B;Sondergaard, I
近红外反射光谱作为筛选方法进行了测试，通过主成分分析 (PCA) 分类来表征大麦收藏中的高赖氨酸突变体。每个簇内样品的平均光谱识别出 2270-2360 nm 区域内的基因特异性模式。发现代表 lys5 基因座（Riso 突变体 13 和 29）的特征光谱特征与淀粉和 (1-->3,1-->4)-β-葡聚糖百分比的巨大变化相关。这些等位基因通过高水平的 (1-->3,1-->4)-β-葡聚糖（高达 15-20%）补偿了低水平的淀粉（低至 30%），从而在正常大麦的范围内保持多糖产量恒定在 50-55%。光谱工具通过独立的数据集进行了测试，其中包含六个具有未知多糖成分的突变体。其中四个的光谱数据被分类在 PCA 中的高 (1-->3,1-->4)-β-葡聚糖 BG lys5 簇内。验证了其高 (1-->3,1-->4)-β-葡聚糖和低淀粉含量。结论是，可以通过光谱文库的化学计量学分类直接从表型发现遗传多样性，例如来自胚乳组织中基因调节的多糖和储存蛋白途径的遗传多样性，代表大麦基因库的数字化表型。 (C) 2004 Elsevier Ltd. 保留所有权利。
Near Infrared Reflectance spectroscopy was tested as a screening method to characterise high lysine mutants from a barley collection by classification through Principal Component Analysis (PCA). Mean spectra of the samples within each cluster identified gene-specific patterns in the 2270-2360 nm region. The characteristic spectral signatures representing the lys5 locus (Riso mutants 13 and 29) were found to be associated with large changes in percentage of starch and (1-->3,1-->4)-beta-glucan. These alleles compensated for a low level of starch (down to 30%) by a high level of (1-->3,1-->4)-beta-glucan (up to 15-20%), thus, maintaining a constant production of polysaccharides at 50-55%, within the range of normal barley.The spectral tool was tested by an independent data set with six mutants with unknown polysaccharide composition. Spectral data from four of these were classified within the high (1-->3,1-->4)-beta-glucan BG lys5 cluster in a PCA. Their high (1-->3,1-->4)-beta-glucan and low starch content was verified. It is concluded that genetic diversity such as from gene regulated polysaccharide and storage protein pathways in the endosperm tissue can be discovered directly from the phenotype by chemometric classification of a spectral library, representing the digitised phenome from a barley gene bank. (C) 2004 Elsevier Ltd. All rights reserved.