Evaluation of profertility effect of probiotic Lactobacillus plantarum 2621 in a murine model.
Evaluation of profertility effect of probiotic Lactobacillus plantarum 2621 in a murine model.
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DOI：
10.4103/0971-5916.162127
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发表时间：
2015-07
期刊：
影响因子：
--
通讯作者：
Prabha V
中科院分区：
文献类型：
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作者：
Bhandari P;Prabha V
细菌性泌尿生殖道感染在育龄女性人群中发病率较高，影响生育。大肠埃希氏菌菌株可以定植于阴道，并取代自然微生物群。乳杆菌是健康女性阴道中的主要微生物，它保持阴道的酸性pH值，从而抑制致病微生物。对乳杆菌的研究表明，这些化合物可以抑制大肠杆菌的生长和阴道定植。抗菌治疗的另一种治疗方法是通过益生菌管理重新建立微生物体内的乳杆菌，以恢复生育能力。因此，本研究的目的是确定具有益生菌特性的植物乳杆菌2621菌株的能力，防止大肠杆菌在阴道内定植引起精子凝集，并在小鼠模型上评价其促生育作用。将筛选出的小鼠分为5组，即对照组、大肠杆菌组、乳酸菌组、预防和治疗组。对照组给予L PBS 20µm，大肠杆菌组给予106cfu/20µm L，益生菌组给予乳酸菌108cfu/20µm L，连续10d。预防性用乳酸菌连续10次阴道定植(108CFU/20微米L)。24 h后阴道感染10天(106cfu/20微克L)，治疗组连续10天阴道定植106cfu/20微克/L，24小时后阴道内注射乳酸菌10天，对照组、益生菌组和治疗组在交配和妊娠期结束时产仔数明显低于预防组和用药组。结果表明，乳杆菌阻断了病原菌的定植，增强了自然菌群，恢复了繁殖力。
Urogenital infections of bacterial origin have a high incidence among the female population at reproductive age, affecting the fertility. Strains of Escherichia coli can colonize the vagina and replace natural microflora. Lactobacillus the predominant vaginal microorganism in healthy women, maintains the acidic vaginal pH which inhibits pathogenic microorganisms. Studies on Lactobacillus have shown that these can inhibit E. coli growth and vaginal colonization. An alternative therapeutic approach to antimicrobial therapy is to re-establish Lactobacillus in this microbiome through probiotic administration to resurge fertility. Therefore, the aim of the present study was to determine the capability of L. plantarum 2621 strain with probiotic properties, to prevent the vaginal colonization of E. coli causing agglutination of sperms and to evaluate its profertility effect in a murine model. Screened mice were divided into five groups i.e. control group, E. coli group, Lactobacillus group, prophylactic and therapeutic groups. The control group was infused with 20 µl PBS, E.coli group was administered with 106 cfu/20 µl E. coli, and probiotic group was administered with Lactobacillus (108 cfu/20 µl) for 10 consecutive days. In prophylactic group, the vagina was colonized with 10 consecutive doses of Lactobacillus (108 cfu/20 µl). After 24 h, it was followed by 10 day intravaginal infection with E. coli (106 cfu/20 µl) whereas for the therapeutic group vagina was colonized with (106 cfu/20 µl) E. coli for 10 consecutive days, followed by 10 day intravaginal administration with Lactobacillus after 24 h. Upon mating and completion of gestation period, control, probiotic and the therapeutic groups had litters in contrast to the prophylactic group and the group administered with E. coli. Results indicated that Lactobacillus intermitted colonization of pathogenic strains that resulted in reinforcement of natural microflora and resurge fertility.