Cardiovascular effects of Nemopilema nomurai (Scyphozoa: Rhizostomeae) jellyfish venom in rats
Cardiovascular effects of Nemopilema nomurai (Scyphozoa: Rhizostomeae) jellyfish venom in rats
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DOI：
10.1016/j.toxlet.2006.09.009
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发表时间：
2006-12-15
影响因子：
3.5
通讯作者：
Hodgson, Wayne C.
中科院分区：
文献类型：
--
作者：
Kim, Euikyung;Lee, Seunghwan;Hodgson, Wayne C.
过去几年，中国、韩国和日本海域的巨型水母Nemopilema nomurai（Scyphozoa：Rhizostomeae）的数量在没有任何明确原因的情况下急剧增加。这对该地区的渔业造成了严重损害。在一项初步研究中，我们观察到野村猪笼草的毒液会导致小鼠功能性心脏抑制。然而，没有研究其作用机制。在本研究中，我们研究了野村猪笼草刺丝囊来源的毒液对麻醉大鼠心血管的影响。毒液（0.1-2.4 mg蛋白质/kg，静脉注射）产生剂量依赖性低血压（累积剂量为3 mg/kg时为初始值的65 +/- 12%）和心动过缓（累积剂量为3 mg/kg时为初始值的80 +/- 5%）。在最高剂量下，其特点是血压短暂下降（第一阶段），然后恢复到基础水平，然后血压缓慢下降（第二阶段）。毒液还降低了大鼠离体心房的收缩率和收缩力。有趣的是，毒液诱导孤立的主动脉环收缩，这种收缩被非洛地平阻断，但不被哌唑嗪阻断，这表明收缩是由钙通道激活介导的。这些结果表明，野村猪笼草毒液的负性肌力和变时作用可能是由于对心脏的直接作用所致。 (c) 2006 Elsevier Ireland Ltd. 保留所有权利。
Over the past few years, populations of the giant jellyfish Nemopilema nomurai (Scyphozoa: Rhizostomeae) have increased dramatically in the waters of China, Korea, and Japan without any definitive reason. This has resulted in severe damage to fisheries in the areas. During a pilot study, we observed that the venom of N. nomurai produced a functional cardiac depression in mice. However, the mechanism of action was not examined. In the present study, we investigated the cardiovascular effects of nematocyst-derived venom from N. nomurai in anesthetized rats. Venom (0.1-2.4 mg protein/kg, i.v.) produced dose-dependent hypotension (65 +/- 12% of initial at a cumulative dose of 3 mg/kg) and bradycardia (80 +/- 5% of initial at a cumulative dose of 3 mg/kg). At the highest dose, this was characterized by a transient decrease in blood pressure (phase 1) followed by a return to basal level and then a slower decrease in blood pressure (phase 2). Venom also produced a decrease in rate and force of contraction in the rat isolated atria. Interestingly, venom induced a contraction of isolated aortic rings which was blocked by felodipine but not by prazosin, suggesting the contraction is mediated by calcium channel activation. These results suggest that the negative inotropic and chronotropic effects of the venom of N. nomurai may be due to a direct effect on the heart. (c) 2006 Elsevier Ireland Ltd. All rights reserved.