Low gut microbiota diversity in early infancy precedes asthma at school age
Low gut microbiota diversity in early infancy precedes asthma at school age
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DOI：
10.1111/cea.12253
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发表时间：
2014-06-01
影响因子：
6.1
通讯作者：
Jenmalm, M. C.
中科院分区：
文献类型：
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作者：
Abrahamsson, T. R.;Jakobsson, H. E.;Jenmalm, M. C.
在生命的第一年，肠道微生物群的低总多样性与婴儿期的过敏性疾病有关，但很少有人知道早期微生物多样性与学龄后期的过敏性疾病有关。目的探讨学龄儿童粪便中微生物多样性及其与哮喘、过敏性鼻结膜炎（ARC）和湿疹等过敏性疾病的关系。方法采用16S rDNA 454焦磷酸测序技术对47名婴儿在1周龄、1月龄和12月龄时的粪便样本进行微生物多样性和组成分析，随后在7岁时评估过敏性疾病和皮肤点刺试验反应性（ClinicalTrials.gov ID NCT01285830）。结果哮喘患儿（n = 8）在1周龄（P = 0.04）和1月龄（P = 0.003）时肠道菌群多样性低于非哮喘患儿（P = 0.003），而过敏性鼻结膜炎（n = 13）、湿疹（n = 12）和皮肤点刺反应阳性（n = 14）在7岁时与肠道菌群多样性无关。哮喘也与婴儿期（12个月）的微生物群组成无关。在婴儿期患有IgE相关湿疹并随后发展为哮喘的儿童的微生物多样性低于那些没有的儿童。然而，在有或没有过敏性疾病的儿童之间，细菌门和属的相对丰度没有显著差异。结论和临床意义出生后第一个月肠道菌群的低多样性与哮喘有关，但与7岁儿童的ARC无关。在出生后第一个月内影响婴儿微生物定植的措施可能会影响儿童期哮喘的发展。
Background Low total diversity of the gut microbiota during the first year of life is associated with allergic diseases in infancy, but little is known how early microbial diversity is related to allergic disease later in school age. Objective To assess microbial diversity and characterize the dominant bacteria in stool during the first year of life in relation to the prevalence of different allergic diseases in school age, such as asthma, allergic rhinoconjunctivitis (ARC) and eczema. Methods The microbial diversity and composition was analysed with barcoded 16S rDNA 454 pyrosequencing in stool samples at 1week, 1month and 12months of age in 47 infants which were subsequently assessed for allergic disease and skin prick test reactivity at 7years of age (ClinicalTrials.gov ID NCT01285830). Results Children developing asthma (n=8) had a lower diversity of the total microbiota than non-asthmatic children at 1week (P=0.04) and 1month (P=0.003) of age, whereas allergic rhinoconjunctivitis (n=13), eczema (n=12) and positive skin prick reactivity (n=14) at 7years of age did not associate with the gut microbiota diversity. Neither was asthma associated with the microbiota composition later in infancy (at 12months). Children having IgE-associated eczema in infancy and subsequently developing asthma had lower microbial diversity than those that did not. There were no significant differences, however, in relative abundance of bacterial phyla and genera between children with or without allergic disease. Conclusion and Clinical Relevance Low total diversity of the gut microbiota during the first month of life was associated with asthma but not ARC in children at 7years of age. Measures affecting microbial colonization of the infant during the first month of life may impact asthma development in childhood.