Overexpression of Peroxiredoxin 6 Protects Neoplastic Cells against Apoptosis in Canine Haemangiosarcoma
Overexpression of Peroxiredoxin 6 Protects Neoplastic Cells against Apoptosis in Canine Haemangiosarcoma
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DOI：
10.1016/j.jcpa.2016.05.002
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发表时间：
2016-07-01
影响因子：
0.8
通讯作者：
Sakai, H.
中科院分区：
文献类型：
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作者：
Anwar, Sh.;Yanai, T.;Sakai, H.
犬血管肉瘤（HSA）与人类血管肉瘤一样，是一种罕见的恶性血管内皮细胞肿瘤，预后不良。过氧化物酶的过氧化物氧还蛋白（PRDX）家族，包括哺乳动物中的六个成员（PRDX 1 -6），可能有助于癌细胞在面对氧化应激时的存活，因为这些蛋白质在癌细胞中表现出频繁的上调。在这项研究中，我们研究了PRDX 6的表达水平在自发产生的主要犬HSAs的免疫组化分析，确定该蛋白的显着表达。与正常犬内皮细胞相比，在HSA细胞系中PRDX 6 mRNA和蛋白均过表达，尽管在不同的HSA细胞系之间观察到一些变化。小干扰RNA诱导的PRDX 6下调促进了HSA细胞系的凋亡。PRDX 6抑制增加这些细胞的细胞毒性的观察表明PRDX 6可能发挥重要的细胞保护作用。(C)2016爱思唯尔有限公司版权所有。
Canine haemangiosarcoma (HSA), like human angiosarcoma, is an uncommon malignant vascular endothelial cell tumour associated with a poor prognosis. The peroxiredoxin (PRDX) family of peroxidases, which comprises six members in mammals (PRDX1-6), might contribute to cancer cell survival in the face of oxidative stress as these proteins exhibit frequent upregulation in cancer cells. In this study, we investigated the expression levels of PRDX6 in spontaneously arising primary canine HSAs by immunohistochemical analysis, identifying marked expression of this protein. Both PRDX6 mRNA and protein were overexpressed in HSA cell lines compared with normal canine endothelial cells, although some variation was observed between the different HSA cell lines. Small interfering RNA-induced downregulation of PRDX6 promoted apoptosis in the HSA cell lines. The observation that PRDX6 suppression increased the cytotoxicity of these cells suggests that PRDX6 might play an important cytoprotective role. (C) 2016 Elsevier Ltd. All rights reserved.