Dissociation of cellular transformation from platelet-derived growth factor independence.
Dissociation of cellular transformation from platelet-derived growth factor independence.
复制标题
细胞转化与血小板衍生生长因子独立性的分离。
DOI：
10.1002/jcp.1041260303
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发表时间：
1986
影响因子：
5.6
通讯作者：
Wharton,W
中科院分区：
文献类型：
--
作者：
Scher,CD;Engle,LJ;Eberenz,WM;Ganguly,K;Wharton,W
将自发转化的BALB/c-3T3细胞株ST2-3T3与不依赖PDGF的BALB/c-3T3细胞株THO2融合，以了解转化是否与PDGF非依赖性协同表达。选择杂交瘤细胞，在含次黄嘌呤-氨基蝶呤-胸腺嘧啶核苷(HAT)和哇巴因的培养液中培养，每种亲本类型的未融合细胞在HAT-哇巴因培养液中被杀死。建立了5个独立分离的ST2-3T3xTHO2杂交瘤细胞系，并对其转化和PDGF反应性进行了鉴定。所有五个细胞都发生了转化，具有无序的生长模式，最终获得了与ST2-3T3细胞相似的细胞密度。其中两个株系对PDGF没有反应：有丝分裂原既没有诱导PDGF调节的溶酶体蛋白的合成，也没有刺激细胞进入S期；一个株系通过合成MEP和DNA来响应PDGF，而另外两个株系合成了MEP而不是DNA。相反，通过将PDGF敏感的未转化的BALB/c-3TC细胞与THO2融合而获得的4个独立分离的细胞系均对PDGF具有MEP和DNA合成反应，且未转化。提示BALB/c-3T3细胞的转化不需要PDGF的非依赖性。
ST2‐3T3, a spontaneously transformed BALB/c‐3T3 cell line which does not require platelet‐derived growth factor (PDGF) for growth, was fused to THO2, a PDGF‐responsive non‐transformed BALB/c‐3T3 cell line, in order to learn whether transformation is expressed coordinately with PDGF independence. Hybrid cells were selected and grown in medium containing both HAT (hypoxanthine‐aminopterin‐thymidine) and ouabain; unfused cells of each parental type were killed in HAT‐ouabain medium. Five independently isolated ST2‐3T3xTHO2hybrid cell lines were established and characterized for both transformation and PDGF responsiveness. All five were transformed, having a disorganized growth pattern and achieving a final cell density similar to that of ST2‐3T3 cells. Two of these lines did not respond to a brief treatment with PDGF: the mitogen neither induced the synthesis of a PDGF‐modulated lysosomal protein (termed MEP), nor stimulated the cells to enter the S phase; one line responded to PDGF by synthesizing both MEP and DNA, whereas two others synthesized MEP but not DNA. In contrast, four independently isolated cell lines obtained by fusing PDGF‐responsive non‐transformed BALB/c‐3TC cells to the THO2line were all PDGF‐responsive for both MEP and DNA synthesis and were not transformed. It appears that PDGF independence is not required for the transformation of BALB/c‐3T3 cells.