Air-Flow-Based Single-Cell Dispensing System
Air-Flow-Based Single-Cell Dispensing System
复制标题
基于气流的单细胞分液系统
DOI：
10.1163/156855311x614572
复制
发表时间：
2012
影响因子：
2
通讯作者：
F. Arai
中科院分区：
文献类型：
--
作者：
T. Kawahara;S. Ohashi;M. Hagiwara;Y. Yamanishi;F. Arai
我们讨论了一种设计和制造方法，以提高单细胞点胶的成功率。将两对电容传感器放置在生物芯片中以检测细胞的流速，并通过同步定时来施加气压以喷射细胞。综合考虑了由气压引起的背压、系统的响应时间、传感器特性和气压引起的点胶延迟等因素，提出了一种综合的设计理论，以最小化系统的扰动，最大化系统的吞吐量。然后，该系统理论上具有以每秒喷射3个细胞的能力，并且最大流速为10 mm/s。该系统的新奇在于生物芯片是一次性的，这与传统的机械喷墨系统不同;因为生物芯片是低成本的并且是一次性的，这防止了污染并且意味着驱动系统是可重复使用的。最后，我们成功地从一个生物芯片的一个聚苯乙烯珠（100 μm）的自动分配到培养孔气氛使用开发的细胞弹射系统，成功率为50%。此外，我们还成功地利用所开发的系统，单猪卵母细胞分配。
We discuss a design and fabrication approach to increase the success rate of single-cell dispensing. Two pairs of capacitance sensors are placed in a biochip to detect the flow velocity of cells and air pressure is applied to eject cells by synchronizing the timing. A comprehensive design theory, which takes into account the back-pressure caused by the air pressure, the response time of the system, the sensor properties and the delay of the dispensing from the air pressure, is developed in order to minimize the disturbance of the system and maximize the throughput of the ejection system. Then, the system theoretically has a capability to eject 3 cells/s and the maximum flow velocity is 10 mm/s. The novelty of the system is that the biochip is disposable, which is unlike the conventional mechanical inkjet system; because the biochip is low cost and disposable this prevents contamination and means the drive system is reusable. Finally, we succeeded in automatic dispensing of a single polystyrene bead (100 μm) from a biochip to a culture well atmosphere using the developed cell ejection system with a success rate of 50%. Furthermore, we also succeeded in single swine oocyte dispensing by using the developed system.
影响因子：
4
作者：
Hagiwara, Masaya;Kawahara, Tomohiro;Arai, Fumihito
通讯作者：
Arai, Fumihito