KCNE2, a down-regulated gene identified by in silico analysis, suppressed proliferation of gastric cancer cells
KCNE2, a down-regulated gene identified by in silico analysis, suppressed proliferation of gastric cancer cells
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KCNE2 是一种通过计算机分析鉴定出的下调基因，可抑制胃癌细胞的增殖。
DOI：
10.1016/j.canlet.2006.02.010
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发表时间：
2007-02-08
期刊：
影响因子：
9.7
通讯作者：
Fan Daiming
中科院分区：
文献类型：
--
作者：
Pan Yanglin;Zhao Lina;Fan Daiming
发现胃癌差异表达基因对于阐明胃肿瘤发生的分子机制具有重要意义。在这里，38个基因在胃癌和正常胃粘膜中的差异表达基因通过电子邮件的方法进行了研究。钾通道蛋白KCNE2被确定为在胃癌中下调的基因，用于进一步研究。我们研究了KCNE2在胃癌组织和细胞系中的表达，并检测了KCNE2对胃癌细胞增殖的影响。KCNE2在胃癌组织和细胞系中的表达明显下调。强迫过表达KCNE2显著抑制SGC7901细胞的生长和细胞周期进程，这可能与Cyclin D1下调有关。KCNE2还能抑制SGC7901细胞在软琼脂中的生长及其在裸鼠体内的致瘤性。综上所述，我们的工作表明，在电子分析中，方法可以被用来有效地识别癌症相关基因。KCNE2作为一种新的胃癌下调基因，具有抑制细胞增殖和抑制胃肿瘤发生的作用。(C)2006爱思唯尔爱尔兰有限公司。保留所有权利。
It is important to identify the differentially expressed gene in gastric cancer for elucidating the molecular mechanisms of tumorigenesis of stomach. Here, 38 genes differentially expressed genes between gastric cancer and normal gastric mucosa by in silico approaches. A potassium channel protein KCNE2, identified as a down-regulated gene in gastric cancer, was chosen for further study. We investigated the expression of KCNE2 in gastric cancer tissues and cell lines and examined the effect of KCNE2 on proliferation of gastric cancer. The expression of KCNE2 was markedly down-regulated in gastric cancer tissues and cell lines. Forced overexpression of KCNE2 suppressed the growth of SGC7901 cells and cell cycle progression significantly, which might be related to the down-regulation of Cyclin D1. KCNE2 also inhibited SGC7901 cell growth in soft agar and its tumorigenicity in nude mice. Taken together, our work showed that in silico analysis approaches could be used to identify cancer-related genes effectively. KCNE2, as a novel down-regulated gene in gastric cancer, suppressed cell proliferation and tumorigenesis of stomach. (c) 2006 Elsevier Ireland Ltd. All rights reserved.