Serum microRNAs as diagnostic markers of endometriosis: a comprehensive array-based analysis
Serum microRNAs as diagnostic markers of endometriosis: a comprehensive array-based analysis
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DOI：
10.1016/j.fertnstert.2016.04.013
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发表时间：
2016-08-01
影响因子：
6.7
通讯作者：
Taylor, Hugh S.
中科院分区：
文献类型：
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作者：
Cosar, Emine;Mamillapalli, Ramanaiah;Taylor, Hugh S.
目的：探讨子宫内膜异位症患者血清中的miRNAs。设计：病例对照研究。地点：大学医院。患者(S)：子宫内膜异位症患者(n=24)和非子宫内膜异位症患者(n=24)。干预(S)：来自经手术诊断的患者的血清样本。主要结果衡量标准(S)：使用非子宫内膜异位症患者的miRNA进行芯片芯片分析，并通过实时定量聚合酶链式反应(qRT-PCR)进行确认。结果：芯片中miR-3613-5p、miR-6755-3p表达下调，miR-125b-5p、miR-150-5p、miR-342-3p、miR-143-3p、miR-145-5p、miR-500a-3p、miR-451a、miR-18a-5p表达上调10倍以上。这些结果得到了qRT-PCR的证实。在差异表达的miRNA中，miR-125b-5p的表达水平在ROC曲线下面积(AUC)最高。当将miR-125b-5p、miR-451a和miR-3613-5p与Logistic回归模型相结合时，AUC值最高可达1.000。结论：我们从血清中分离出了几个可以区分子宫内膜异位症和非子宫内膜异位症的miRNA。MIR-125b-5p作为单一诊断生物标志物的潜力最大。该miRNA与miR-451a和miR-3613-5p的结合进一步提高了诊断性能。(C)2016年由美国生殖医学会评选。
Objective: To investigate serum microRNAs (miRNAs) in women with endometriosis.Design: Case-control study.Setting: University hospital.Patient(s): Women with (n = 24) and without (n = 24) endometriosis.Intervention(s): Serum samples were obtained from surgically diagnosed subjects.Main Outcome Measure(s): miRNA from women with without endometriosis were used for microarray profiling and confirmed by means of quantitative real-time polymerase chain reaction (qRT-PCR). Receiver operating characteristic (ROC) analysis was performed on differentially expressed miRNAs.Result(s): miR-3613-5p, miR-6755-3p were down-regulated and miR-125b-5p, miR-150-5p, miR-342-3p, miR-143-3p, miR-145-5p, miR-500a-3p, miR-451a, miR-18a-5p were up-regulated more than 10-fold in the microarray. These results were confirmed with the use of qRT-PCR. Among the differentially expressed miRNAs, miR-125b-5p expression levels had the highest area under the ROC curve (AUC). The maximum AUC score of 1.000 was achieved when combining miR-125b-5p, miR-451a, and miR-3613-5p with the use of a logistic regression model.Conclusion(s): We identified several miRNAs in serum that distinguished subjects with endometriosis from those without. miR-125b-5p had the greatest potential as a single diagnostic biomarker. A combination of that miRNA with miR-451a and miR-3613-5p further improved diagnostic performance. (C) 2016 by American Society for Reproductive Medicine.