ARE THE 5' ENDS OF INFLUENZA VIRAL MESSENGER-RNAS SYNTHESIZED INVIVO DONATED BY HOST MESSENGER-RNAS
ARE THE 5' ENDS OF INFLUENZA VIRAL MESSENGER-RNAS SYNTHESIZED INVIVO DONATED BY HOST MESSENGER-RNAS
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DOI：
10.1016/0092-8674(79)90052-7
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发表时间：
1979-01-01
期刊：
影响因子：
64.5
通讯作者：
BOULOY, M
中科院分区：
文献类型：
--
作者：
KRUG, RM;BRONI, BA;BOULOY, M
真核mRNA作为流感病毒RNA体外转录的引物，并将其5“末端甲基化帽和一小段内部核苷酸贡献给所得的病毒RNA转录物。如果类似的机制在体内起作用，那么在感染细胞中合成的病毒mRNA将在其5“末端包含一小段核苷酸，包括帽，这不是病毒编码的。本研究旨在确定体内病毒mRNA中是否存在这样的非病毒序列。凝胶电泳分析表明，体内病毒mRNA的5“端片段比体外合成的ApG引物互补RNA的5”端片段长10-15个核苷酸。由于后一个片段恰好起始于病毒体RNA模板的3“末端，因此该结果表明体内病毒mRNA的片段在其5”末端含有10-15个核苷酸，这些核苷酸不是病毒编码的。当3 H-甲基标记的体内病毒mRNA与病毒体RNA杂交时，mRNA的5“末端帽结构不受胰腺或T1 RNA酶消化的保护。病毒mRNA每条链上发现的3个6-甲基腺苷（m6 A）残基之一也没有受到保护。显然，宿主细胞mRNA作为病毒RNA在感染细胞中转录的引物，它们将它们的帽和10-15个内部核苷酸（其中一个是m6 A）贡献给所产生的病毒mRNA分子。
Eukaryotic mRNA serve as primers for influenza viral RNA transcription in vitro and donate their 5'' terminal methylated cap and a short stretch of internal nucleotides to the resulting viral RNA transcripts. If a similar mechanism operates in vivo, then the viral mRNA synthesized in the infected cell would contain a short stretch of nucleotides at its 5'' end, including the cap, which is not viral-coded. This study was undertaken to establish whether such a nonviral sequence exists in in vivo viral mRNA. Gel electrophoresis analysis indicated that the segments of in vivo viral mRNA were 10-15 nucleotides longer at their 5'' end than the segments of ApG-primed complementary RNA synthesized in vitro. As the latter segments initiate exactly at the 3'' end of the virion RNA templates, this result indicates that the segments of in vivo viral mRNA contain 10-15 nucleotides at their 5'' end which are not viral-coded. When 3H-methyl-labeled in vivo viral mRNA was hybridized to virion RNA, the 5'' terminal cap structure of the mRNA was not protected against pancreatic or T1 RNase digestion. One of the 3 6-methyladenosine (m6A) residues found per chain of viral mRNA was also not protected. Apparently host cell mRNA serve as primers for viral RNA transcription in the infected cell, and they donate their cap and 10-15 internal nucleotides, one of which is m6A, to the resulting viral mRNA molecules.