Immunoglobulin G locus events in soft tissue sarcoma cell lines.
Immunoglobulin G locus events in soft tissue sarcoma cell lines.
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软组织肉瘤细胞系中的免疫球蛋白 G 位点事件
DOI：
10.1371/journal.pone.0021276
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Gu J
中科院分区：
文献类型：
--
作者：
Chen Z;Li J;Xiao Y;Zhang J;Zhao Y;Liu Y;Ma C;Qiu Y;Luo J;Huang G;Korteweg C;Gu J
最近发现B淋巴细胞以外的细胞也表达免疫球蛋白(Igs)，包括各种人类癌细胞。肉瘤来源于间充质，关于肉瘤细胞中 Ig 产生的知识非常有限。在这里，我们研究了 3 种肉瘤细胞系中免疫球蛋白 G (IgG) 表达的现象及其分子基础。 RT-PCR检测IgG重链和κ轻链的mRNA转录物。此外，通过Western blot和免疫荧光证实了IgG蛋白的表达。免疫电子显微镜将 IgG 定位于细胞膜和粗面内质网。在这些肉瘤细胞中还鉴定出了基因重排和类别转换重组所需的必需酶以及 IgG 种系转录本。染色质免疫沉淀结果表明重组激活基因和 Ig 重链调节元件均发生组蛋白 H3 乙酰化。总的来说，这些结果证实了肉瘤细胞中存在 IgG 表达，其机制与调节 B 淋巴细胞中 IgG 表达的机制非常相似。
Recently immunoglobulins (Igs) have been found to be expressed by cells other than B lymphocytes, including various human carcinoma cells. Sarcomas are derived from mesenchyme, and the knowledge about the occurrence of Ig production in sarcoma cells is very limited. Here we investigated the phenomenon of immunoglobulin G (IgG) expression and its molecular basis in 3 sarcoma cell lines. The mRNA transcripts of IgG heavy chain and kappa light chain were detected by RT-PCR. In addition, the expression of IgG proteins was confirmed by Western blot and immunofluorescence. Immuno-electron microscopy localized IgG to the cell membrane and rough endoplasmic reticulum. The essential enzymes required for gene rearrangement and class switch recombination, and IgG germ-line transcripts were also identified in these sarcoma cells. Chromatin immunoprecipitation results demonstrated histone H3 acetylation of both the recombination activating gene and Ig heavy chain regulatory elements. Collectively, these results confirmed IgG expression in sarcoma cells, the mechanism of which is very similar to that regulating IgG expression in B lymphocytes.